Detailed information of TA system TA036215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2413468..2414100
• Replicon: chromosome
• Accession: NZ_AP022579
• Organism: Mycolicibacterium boenickei strain JCM 15653

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: G6N57_RS11445
• Protein ID: WP_077740512.1
• Coordinates: 2413651..2414100 (+)
• Length: 150 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: A0A7I7N5G7
• Locus tag: G6N57_RS11440
• Protein ID: WP_036447720.1
• Coordinates: 2413468..2413647 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N57_RS11425	2409106..2409840	+	735	WP_077740509.1	enoyl-CoA hydratase	-
G6N57_RS11430	2409842..2410957	+	1116	WP_077740510.1	MBL fold metallo-hydrolase	-
G6N57_RS11435	2411024..2413399	+	2376	WP_077740511.1	cation-translocating P-type ATPase	-
G6N57_RS11440	2413468..2413647	+	180	WP_036447720.1	antitoxin	Antitoxin
G6N57_RS11445	2413651..2414100	+	450	WP_077740512.1	SRPBCC family protein	Toxin
G6N57_RS11450	2414155..2414529	-	375	WP_077740513.1	fumarate reductase subunit FrdD	-
G6N57_RS11455	2414511..2414885	-	375	WP_077740514.1	fumarate reductase subunit C	-
G6N57_RS11460	2414882..2415625	-	744	WP_077740515.1	succinate dehydrogenase/fumarate reductase iron-sulfur subunit	-
G6N57_RS11465	2415627..2417369	-	1743	WP_077740516.1	fumarate reductase (quinol) flavoprotein subunit	-
G6N57_RS11470	2417379..2417621	-	243	WP_075919415.1	hypothetical protein	-
G6N57_RS11475	2417790..2419010	-	1221	WP_077740517.1	cytochrome P450	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 150 a.a.
• Molecular weight: 16086.66 Da
• Isoelectric Point: 9.8774

>T36215 WP_077740512.1 NZ_AP022579:2413651-2414100 [Mycolicibacterium boenickei]
MAKLSVSVDVPLPPEKAWEYASDLSRYDEWLSIHRAWRSKLPETLEKGTVIDSIVEVKGMLNRVKWTLVNYKPPQSLTLN
GDGRGGVKVKLIGKIAPAQVDGGEGAKVSFDVHLGGPALFGPIGMVVAAALKGDIQQSLNKFKALYASS

Nucleotide

• Length: 450 bp

>T36215 NZ_AP022579:2413651-2414100 [Mycolicibacterium boenickei]
ATGGCTAAATTGTCTGTCTCCGTCGATGTTCCATTGCCGCCGGAGAAGGCGTGGGAGTACGCCTCTGATCTGTCCCGGTA
CGACGAGTGGCTCAGCATCCACCGCGCCTGGCGGTCGAAGTTGCCCGAGACCCTGGAAAAGGGCACGGTCATCGACTCGA
TCGTCGAAGTCAAGGGCATGCTGAACCGTGTGAAGTGGACGCTGGTGAATTACAAGCCACCGCAGTCCCTGACCCTCAAC
GGCGATGGCCGTGGCGGGGTAAAGGTCAAGCTGATCGGAAAGATCGCTCCGGCCCAGGTTGACGGCGGCGAGGGCGCGAA
AGTGTCCTTCGACGTGCATCTAGGAGGGCCTGCCCTGTTCGGGCCGATCGGCATGGTGGTCGCGGCCGCGCTCAAGGGCG
ATATCCAGCAGTCGCTGAACAAGTTCAAGGCGCTCTACGCGTCGTCGTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6392.07 Da
• Isoelectric Point: 4.4883

>AT36215 WP_036447720.1 NZ_AP022579:2413468-2413647 [Mycolicibacterium boenickei]
MGFLDKAKDLLSQNADKVEQAIDKAGDIVDEKTQGKYSGAVDKAQEAAKNAINKEEPQQ

Nucleotide

• Length: 180 bp

>AT36215 NZ_AP022579:2413468-2413647 [Mycolicibacterium boenickei]
ATGGGATTCCTGGACAAGGCCAAGGACCTTCTGTCTCAGAACGCGGACAAGGTAGAGCAGGCCATCGACAAGGCCGGCGA
CATCGTCGACGAGAAGACGCAGGGCAAGTACTCCGGCGCGGTCGACAAGGCACAGGAAGCGGCGAAGAACGCCATCAACA
AGGAAGAACCGCAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7N5G7