Detailed information of TA system TA036209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 3112240..3112826
• Replicon: chromosome
• Accession: NZ_AP022575
• Organism: Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G6N20_RS14065
• Protein ID: WP_083048016.1
• Coordinates: 3112240..3112584 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G6N20_RS14070
• Protein ID: WP_083048040.1
• Coordinates: 3112578..3112826 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N20_RS14060	3110910..3112136	-	1227	WP_083048018.1	NarK/NasA family nitrate transporter	-
G6N20_RS14065	3112240..3112584	-	345	WP_083048016.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N20_RS14070	3112578..3112826	-	249	WP_083048040.1	ribbon-helix-helix protein, CopG family	Antitoxin
G6N20_RS14075	3112899..3113377	-	479	Protein_2781	DUF222 domain-containing protein	-
G6N20_RS14080	3113589..3115964	+	2376	WP_083048014.1	flippase-like domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12156.09 Da
• Isoelectric Point: 10.4096

>T36209 WP_083048016.1 NZ_AP022575:c3112584-3112240 [Mycobacterium shinjukuense]
VVIGRAEIYWADLGSPSGSRPAKRRPVLVIQSDPYNASRLATVLAAVITSNTALAAMPGNVFLPAATTRLPRDSVVNITA
LVTLNKTDLTDQVGELPPSLMHEVDRGLRRVLGL

Nucleotide

• Length: 345 bp

>T36209 NZ_AP022575:c3112584-3112240 [Mycobacterium shinjukuense]
GTGGTGATTGGCCGCGCTGAGATCTACTGGGCCGACCTTGGGTCGCCGTCCGGCAGCCGGCCCGCCAAGCGCCGCCCGGT
GCTCGTGATCCAGTCCGATCCGTACAACGCCAGTCGCCTTGCGACCGTGCTCGCGGCGGTGATCACGTCCAATACTGCCC
TGGCAGCAATGCCCGGGAACGTGTTCTTGCCCGCAGCCACAACGAGACTGCCACGTGATTCGGTCGTCAACATCACAGCG
CTTGTCACGCTCAACAAGACCGACCTCACCGATCAGGTCGGGGAGCTGCCGCCGAGCTTGATGCATGAAGTTGACCGGGG
ACTCCGACGCGTGCTGGGCCTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8970.81 Da
• Isoelectric Point: 4.1237

>AT36209 WP_083048040.1 NZ_AP022575:c3112826-3112578 [Mycobacterium shinjukuense]
VKTAISLPDETFDRVSRRASDLGMSRSEFFTRAAQRYLDELDAQALTGQIDSALESLRGTDESESAAVAAGYRVLAAVDD
EW

Nucleotide

• Length: 249 bp

>AT36209 NZ_AP022575:c3112826-3112578 [Mycobacterium shinjukuense]
GTGAAGACCGCTATCTCCCTGCCGGACGAGACGTTCGATCGTGTATCCCGGCGTGCGAGTGACCTCGGCATGAGTCGGTC
TGAGTTCTTCACCCGGGCTGCACAGCGCTACTTGGACGAGCTGGATGCCCAAGCGCTGACCGGACAGATCGACAGTGCTC
TCGAGAGCCTGCGTGGCACCGACGAATCGGAGTCAGCCGCCGTGGCCGCTGGCTACCGCGTGTTAGCGGCCGTGGACGAT
GAGTGGTGA