Detailed information of TA system TA036205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 2736081..2736781
• Replicon: chromosome
• Accession: NZ_AP022575
• Organism: Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N20_RS12390
• Protein ID: WP_142271978.1
• Coordinates: 2736081..2736494 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N20_RS12395
• Protein ID: WP_083047441.1
• Coordinates: 2736488..2736781 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N20_RS12365	2731819..2732988	-	1170	WP_083047429.1	acyl-CoA dehydrogenase	-
G6N20_RS12370	2733002..2733517	-	516	WP_083047431.1	5-(carboxyamino)imidazole ribonucleotide mutase	-
G6N20_RS12375	2733514..2734779	-	1266	WP_083047433.1	5-(carboxyamino)imidazole ribonucleotide synthase	-
G6N20_RS12380	2734879..2735553	+	675	WP_083047435.1	GtrA family protein	-
G6N20_RS12385	2735508..2736026	-	519	WP_083047437.1	PH domain-containing protein	-
G6N20_RS12390	2736081..2736494	-	414	WP_142271978.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N20_RS12395	2736488..2736781	-	294	WP_083047441.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
G6N20_RS12400	2736771..2737574	-	804	WP_083047443.1	biotin--[acetyl-CoA-carboxylase] ligase	-
G6N20_RS12405	2737631..2739271	+	1641	WP_083047445.1	acyl-CoA carboxylase subunit beta	-
G6N20_RS12410	2739252..2739650	+	399	Protein_2452	acyl-CoA carboxylase subunit epsilon	-
G6N20_RS12415	2739647..2740288	+	642	WP_083047447.1	septum formation inhibitor Maf	-
G6N20_RS12420	2740490..2740786	+	297	Protein_2454	transposase family protein	-
G6N20_RS12425	2740786..2741513	-	728	Protein_2455	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2736081..2751805	15724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-130)

Antitoxin

• Post-segregation antitoxin CcdA (26-67)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14813.05 Da
• Isoelectric Point: 6.6410

>T36205 WP_142271978.1 NZ_AP022575:c2736494-2736081 [Mycobacterium shinjukuense]
IVRAGQASVDEVVVDASVLVELLGDFTHGQAVQQRLRDTRLHAPAHLDAEVLSAMGRLHRAGTIDATDVEAGLNRLAQLP
VQRHGLNGLLLGAWARRADVRLLDALYVELASQLRVPLLTVDRKLARVCGMAEDITA

Nucleotide

• Length: 414 bp

>T36205 NZ_AP022575:c2736494-2736081 [Mycobacterium shinjukuense]
ATCGTGAGAGCCGGGCAAGCCTCGGTCGACGAGGTTGTGGTTGACGCGTCCGTCCTGGTGGAACTGCTAGGTGACTTCAC
CCATGGACAGGCAGTACAGCAACGCCTCCGCGACACCCGCCTGCACGCGCCGGCGCATCTGGACGCTGAGGTCTTATCGG
CCATGGGACGGCTGCACCGCGCGGGAACGATCGACGCCACCGACGTTGAGGCCGGGCTTAACCGTCTTGCTCAACTGCCC
GTGCAACGGCATGGCCTCAACGGCCTCCTCCTTGGCGCATGGGCACGACGTGCCGACGTCCGGCTTCTCGATGCGCTCTA
TGTCGAGCTGGCCAGTCAGCTGCGCGTTCCTCTGCTCACCGTCGACCGCAAGCTCGCGCGTGTTTGCGGCATGGCTGAAG
ACATCACTGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10927.32 Da
• Isoelectric Point: 7.2456

>AT36205 WP_083047441.1 NZ_AP022575:c2736781-2736488 [Mycobacterium shinjukuense]
MCANRRARLARPHPRHECVYSVRIARLNVYVPDELADRVRSADVNVSAVVQAALAEELDRRATNAWLDALPPLRGRRSHE
AAIQALDEVRDEFGEPS

Nucleotide

• Length: 294 bp

>AT36205 NZ_AP022575:c2736781-2736488 [Mycobacterium shinjukuense]
ATGTGCGCTAACCGGCGGGCGCGCCTGGCGCGGCCGCACCCCCGGCATGAATGTGTGTATAGTGTGCGTATCGCGAGACT
CAATGTGTACGTTCCAGACGAACTTGCGGACCGGGTGCGCTCCGCCGACGTCAACGTGTCTGCAGTGGTGCAAGCAGCGC
TTGCGGAGGAGCTCGACCGCAGAGCGACCAACGCCTGGCTCGACGCGCTTCCGCCGCTGCGTGGTAGAAGATCGCATGAA
GCGGCGATACAAGCGCTCGATGAGGTTCGCGACGAGTTCGGCGAGCCATCGTGA