Detailed information of TA system TA036203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2489157..2489689
• Replicon: chromosome
• Accession: NZ_AP022575
• Organism: Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G6N20_RS11210
• Protein ID: WP_083046161.1
• Coordinates: 2489390..2489689 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G6N20_RS11205
• Protein ID: WP_083046162.1
• Coordinates: 2489157..2489390 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N20_RS11180	2485525..2485992	-	468	WP_083046165.1	DUF5130 domain-containing protein	-
G6N20_RS11185	2485979..2486212	-	234	WP_083046239.1	hypothetical protein	-
G6N20_RS11190	2486286..2486957	-	672	WP_083046164.1	HNH endonuclease	-
G6N20_RS11195	2487080..2487481	+	402	WP_158084737.1	globin	-
G6N20_RS11200	2487481..2489106	+	1626	WP_142271848.1	glycoside hydrolase family 13 protein	-
G6N20_RS11205	2489157..2489390	+	234	WP_083046162.1	antitoxin	Antitoxin
G6N20_RS11210	2489390..2489689	+	300	WP_083046161.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N20_RS11215	2489707..2490363	-	657	WP_083046237.1	hypothetical protein	-
G6N20_RS11220	2490360..2490776	-	417	WP_083046160.1	acyl-CoA thioesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-98)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10646.40 Da
• Isoelectric Point: 8.0526

>T36203 WP_083046161.1 NZ_AP022575:2489390-2489689 [Mycobacterium shinjukuense]
MRPIQLAHLDKTRPVVILTRELVRPHLARVTVAPITGTIRGLSTEVIVGPANGLQQTSVISCDNIATIPTSALGRQLGYL
LPDQEPALSQAIRAAFDLE

Nucleotide

• Length: 300 bp

>T36203 NZ_AP022575:2489390-2489689 [Mycobacterium shinjukuense]
ATGCGGCCCATCCAGCTGGCTCATCTCGACAAGACCCGGCCCGTCGTGATCCTGACCCGCGAACTCGTCAGACCTCACCT
CGCCCGGGTAACCGTGGCACCCATCACCGGCACCATTCGCGGTCTGTCCACCGAGGTCATTGTCGGTCCAGCCAATGGGC
TGCAGCAGACTTCGGTAATCAGCTGCGACAACATCGCTACCATTCCAACCAGTGCGTTGGGACGCCAGCTCGGCTATCTG
CTACCCGATCAGGAACCGGCCCTCTCGCAAGCGATCCGCGCGGCATTCGACCTCGAGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8615.86 Da
• Isoelectric Point: 4.8485

>AT36203 WP_083046162.1 NZ_AP022575:2489157-2489390 [Mycobacterium shinjukuense]
MSLQIAVRLPEDVVAFIDREVSEDRAPSRAAVVLRALERERRRQIAARDAAILAVTEPDRDLDKLAEHVAKLAVDID

Nucleotide

• Length: 234 bp

>AT36203 NZ_AP022575:2489157-2489390 [Mycobacterium shinjukuense]
ATGAGCCTTCAGATCGCCGTGCGGCTACCCGAGGATGTCGTCGCCTTCATCGATCGCGAGGTCAGCGAGGACCGGGCACC
CAGCCGCGCCGCGGTCGTATTGCGAGCCCTCGAACGCGAACGGCGCCGCCAAATCGCCGCCCGCGACGCCGCGATTCTCG
CCGTCACCGAACCCGATCGCGACCTCGACAAACTCGCCGAACACGTCGCGAAGCTAGCCGTTGACATCGATTGA