Detailed information of TA system TA036202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 2391511..2392115
• Replicon: chromosome
• Accession: NZ_AP022575
• Organism: Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N20_RS10725
• Protein ID: WP_083052148.1
• Coordinates: 2391511..2391903 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7MQB7
• Locus tag: G6N20_RS10730
• Protein ID: WP_042915533.1
• Coordinates: 2391900..2392115 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N20_RS10700	2387542..2388356	+	815	Protein_2113	ExeA family protein	-
G6N20_RS10705	2388595..2389479	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
G6N20_RS10710	2389482..2390369	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
G6N20_RS10715	2390679..2391224	-	546	WP_083052151.1	DUF1802 family protein	-
G6N20_RS10720	2391221..2391490	-	270	WP_083052150.1	DUF2277 family protein	-
G6N20_RS10725	2391511..2391903	-	393	WP_083052148.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N20_RS10730	2391900..2392115	-	216	WP_042915533.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G6N20_RS10735	2392162..2392911	+	750	WP_083052145.1	enoyl-CoA hydratase	-
G6N20_RS10740	2392925..2394007	-	1083	WP_083052142.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
G6N20_RS10745	2394000..2395313	-	1314	WP_083052139.1	ABC transporter substrate-binding protein	-
G6N20_RS10750	2395316..2396143	-	828	WP_083052136.1	carbohydrate ABC transporter permease	-
G6N20_RS10755	2396143..2397051	-	909	WP_142272210.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-45)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14640.77 Da
• Isoelectric Point: 6.7594

>T36202 WP_083052148.1 NZ_AP022575:c2391903-2391511 [Mycobacterium shinjukuense]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATTVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T36202 NZ_AP022575:c2391903-2391511 [Mycobacterium shinjukuense]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGTTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGACGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7541.58 Da
• Isoelectric Point: 4.9732

>AT36202 WP_042915533.1 NZ_AP022575:c2392115-2391900 [Mycobacterium shinjukuense]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMTAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT36202 NZ_AP022575:c2392115-2391900 [Mycobacterium shinjukuense]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGA
CCGCGGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7MQB7