Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) abiEi-abiEii/AbiEii-AbiEi
Location 2388595..2390369 Replicon chromosome
Accession NZ_AP022575
Organism Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)


Gene name abiEi Uniprot ID P71626
Locus tag G6N20_RS10705 Protein ID WP_003414409.1
Coordinates 2388595..2389479 (-) Length 295 a.a.

Antitoxin (Protein)


Gene name abiEii Uniprot ID P71625
Locus tag G6N20_RS10710 Protein ID WP_003414414.1
Coordinates 2389482..2390369 (-) Length 296 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6N20_RS10685 2385002..2385223 - 222 WP_179961464.1 helix-turn-helix domain-containing protein -
G6N20_RS20650 2385696..2386079 + 384 WP_179961554.1 hypothetical protein -
G6N20_RS10695 2386134..2387545 + 1412 Protein_2112 DDE-type integrase/transposase/recombinase -
G6N20_RS10700 2387542..2388356 + 815 Protein_2113 ExeA family protein -
G6N20_RS10705 2388595..2389479 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
G6N20_RS10710 2389482..2390369 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin Antitoxin
G6N20_RS10715 2390679..2391224 - 546 WP_083052151.1 DUF1802 family protein -
G6N20_RS10720 2391221..2391490 - 270 WP_083052150.1 DUF2277 family protein -
G6N20_RS10725 2391511..2391903 - 393 WP_083052148.1 type II toxin-antitoxin system VapC family toxin -
G6N20_RS10730 2391900..2392115 - 216 WP_042915533.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
G6N20_RS10735 2392162..2392911 + 750 WP_083052145.1 enoyl-CoA hydratase -
G6N20_RS10740 2392925..2394007 - 1083 WP_083052142.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
G6N20_RS10745 2394000..2395313 - 1314 WP_083052139.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2383119..2384852 1733


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(36-272)

Antitoxin

AbiEi antitoxin C-terminal domain

(84-237)


Sequences

Toxin        


Download         Length: 295 a.a.        Molecular weight: 33056.72 Da        Isoelectric Point: 7.1520

>T36201 WP_003414409.1 NZ_AP022575:c2389479-2388595 [Mycobacterium shinjukuense]
VAGLTRALVARHALGRAEAYDAALLDVAQDHLLYLLSQTVQFGDNRLVFKGGTSLRKCRLGNVGRFSTDLDFSAPDDEVV
LEVCELIDGARVGGFEFGVQSTRGDGRHWQLRVRHTELGEPRIVASVEFARRPLALPSELLAFIQLPIHKAYGFGLPTLP
VVAEAEACAEKLARYRRVALARDLYDLNHFASRTIDEPLVRRLWVLKVWGDVVDDRRGTRPLRVEDVLAARSEHDFQPDS
IGVLTRPVAMAAWEARVRKRFAFLTDLDADEQRWAACDERHRREVENALAVLRS

Download         Length: 885 bp

>T36201 NZ_AP022575:c2389479-2388595 [Mycobacterium shinjukuense]
GTGGCCGGTCTGACCCGTGCGCTCGTTGCGCGTCACGCTCTTGGTCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGTCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGCGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCATTGGCAGTTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGTCGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCTTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCGGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
GAACCACTTCGCCTCGCGAACGATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACTCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGAAGCGATTTGCGTTCCTCACCGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGTGCTGCGGT
CCTGA

Antitoxin


Download         Length: 296 a.a.        Molecular weight: 32315.19 Da        Isoelectric Point: 9.4723

>AT36201 WP_003414414.1 NZ_AP022575:c2390369-2389482 [Mycobacterium shinjukuense]
VVSPAGADRRIPTWASRVVSGLARDRPVVVTKEDLTQRLTEAGCGRDPDSAIRELRRIGWLVQLPVKGTWAFIPPGEAAI
SDPYLPLRSWLARDQNAGFMLAGASAAWHLGYLDRQPDGRIPIWLPPAKRLPDGLASYVSVVRIPWNAADTALLAPRPAL
LVRRRLDLVAWATGLPALGPEALLVQIATRPASFGPWADLVPHLDDLVADCSDERLERLLSGRPTSAWQRASYLLDSGGE
PARGQALLAKRHTEVMPVTRFTTAHSRDRGESVWAPEYQLVDELVVPLLRVIGKA

Download         Length: 888 bp

>AT36201 NZ_AP022575:c2390369-2389482 [Mycobacterium shinjukuense]
GTGGTGAGCCCAGCCGGCGCCGATCGGCGCATCCCCACGTGGGCGTCACGGGTGGTGTCCGGCCTCGCTCGCGATCGGCC
CGTAGTCGTCACGAAAGAGGATCTGACGCAACGGCTTACAGAGGCTGGGTGTGGACGCGACCCCGACTCCGCCATCCGCG
AACTGCGCCGCATTGGGTGGCTGGTCCAACTGCCGGTGAAAGGCACCTGGGCATTCATTCCCCCCGGCGAGGCCGCCATC
TCGGACCCGTATCTACCGCTGCGGTCGTGGCTGGCCCGTGACCAGAACGCGGGCTTCATGCTGGCCGGCGCATCCGCAGC
GTGGCACCTCGGATACCTGGACCGCCAACCCGACGGCCGCATCCCGATCTGGCTGCCGCCGGCCAAAAGGCTGCCCGACG
GCCTAGCATCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCGGACACCGCACTGCTGGCTCCGCGTCCCGCGTTG
CTGGTCCGGCGGCGGCTCGACCTCGTCGCGTGGGCGACCGGGTTACCGGCGCTAGGCCCAGAAGCATTACTTGTGCAAAT
CGCCACGCGCCCGGCCTCGTTCGGGCCGTGGGCCGACCTTGTCCCCCACCTTGACGACCTCGTCGCCGACTGCAGTGACG
AGCGCCTGGAGCGTCTACTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACTCGGGCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGGCACACCGAAGTGATGCCGGTGACTCGATTCACCACCGCGCACAGCCG
CGACCGTGGCGAGAGTGTCTGGGCTCCCGAGTATCAGCTTGTCGACGAGCTCGTCGTACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y56

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBU9

References