36200

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2363212..2363782 Replicon chromosome
Accession NZ_AP022575
Organism Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag G6N20_RS10590 Protein ID WP_003414166.1
Coordinates 2363212..2363568 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag G6N20_RS10595 Protein ID WP_003901465.1
Coordinates 2363552..2363782 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6N20_RS10570 2358661..2360349 - 1689 WP_083051410.1 alpha/beta hydrolase family protein -
G6N20_RS10575 2360353..2360679 - 327 WP_083051408.1 hypothetical protein -
G6N20_RS10580 2360852..2361436 + 585 WP_083051406.1 DUF3558 domain-containing protein -
G6N20_RS10585 2361461..2363110 + 1650 WP_083051404.1 CocE/NonD family hydrolase -
G6N20_RS10590 2363212..2363568 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
G6N20_RS10595 2363552..2363782 - 231 WP_003901465.1 antitoxin MazE Antitoxin
G6N20_RS10600 2363825..2364868 - 1044 WP_083051402.1 DUF2293 domain-containing protein -
G6N20_RS10605 2365013..2365282 - 270 WP_083051399.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
G6N20_RS10610 2365325..2365600 + 276 WP_042915503.1 DUF1778 domain-containing protein -
G6N20_RS20790 2365945..2366100 + 156 WP_197745506.1 histone acetyltransferase -
G6N20_RS10620 2366170..2366534 + 365 Protein_2097 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T36200 WP_003414166.1 NZ_AP022575:c2363568-2363212 [Mycobacterium shinjukuense]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T36200 NZ_AP022575:c2363568-2363212 [Mycobacterium shinjukuense]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTTCATCTCGACTTATGGTCGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT36200 WP_003901465.1 NZ_AP022575:c2363782-2363552 [Mycobacterium shinjukuense]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT36200 NZ_AP022575:c2363782-2363552 [Mycobacterium shinjukuense]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FBW3

References