Detailed information of TA system TA036198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2327609..2328237
• Replicon: chromosome
• Accession: NZ_AP022575
• Organism: Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N20_RS10385
• Protein ID: WP_083050160.1
• Coordinates: 2327609..2328025 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N20_RS10390
• Protein ID: WP_083050180.1
• Coordinates: 2328022..2328237 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N20_RS10355	2322657..2323559	-	903	WP_083050184.1	4-hydroxy-tetrahydrodipicolinate synthase	-
G6N20_RS10360	2323675..2324427	-	753	WP_083050182.1	FAD-dependent thymidylate synthase	-
G6N20_RS10365	2324477..2324857	+	381	WP_158084776.1	hypothetical protein	-
G6N20_RS10370	2324893..2325135	+	243	WP_197745473.1	hypothetical protein	-
G6N20_RS10375	2325132..2325569	+	438	WP_083050163.1	PIN domain-containing protein	-
G6N20_RS10380	2325663..2327288	-	1626	WP_083050181.1	type I restriction-modification system subunit M	-
G6N20_RS10385	2327609..2328025	-	417	WP_083050160.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
G6N20_RS10390	2328022..2328237	-	216	WP_083050180.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
G6N20_RS10395	2328312..2328707	-	396	WP_083050159.1	type II toxin-antitoxin system VapC family toxin	-
G6N20_RS10400	2328704..2328987	-	284	Protein_2054	type II toxin-antitoxin system VapB family antitoxin	-
G6N20_RS10405	2328965..2330047	-	1083	WP_083050178.1	restriction endonuclease subunit S	-
G6N20_RS10410	2330047..2330175	-	129	Protein_2056	winged helix-turn-helix domain-containing protein	-
G6N20_RS10415	2330459..2330944	-	486	WP_083050156.1	dihydrofolate reductase	-
G6N20_RS10420	2331006..2331806	-	801	WP_083050153.1	thymidylate synthase	-
G6N20_RS10425	2332069..2332809	+	741	WP_083050149.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-39)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15772.96 Da
• Isoelectric Point: 6.8457

>T36198 WP_083050160.1 NZ_AP022575:c2328025-2327609 [Mycobacterium shinjukuense]
MTTRFLLDKSAVYRAHIPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDAVWDRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGISVMHYDQDFERIAGITRQPVEWVVPPGTA

Nucleotide

• Length: 417 bp

>T36198 NZ_AP022575:c2328025-2327609 [Mycobacterium shinjukuense]
ATGACCACTCGCTTTTTGCTCGACAAATCAGCGGTGTACCGCGCTCACATCCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGTGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGCTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACGCGGTGTGGGATCGCGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCATCGCTCGGTCAAGATCCCGGACTTGATTATCGCGGCGGTCGCCGAGCATCA
CGGCATATCGGTCATGCACTACGATCAAGACTTCGAACGCATCGCTGGCATCACACGGCAACCCGTGGAATGGGTGGTGC
CCCCAGGAACGGCATGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7650.62 Da
• Isoelectric Point: 5.0041

>AT36198 WP_083050180.1 NZ_AP022575:c2328237-2328022 [Mycobacterium shinjukuense]
MIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGIEDEALVDTMWRDGHPANTA

Nucleotide

• Length: 216 bp

>AT36198 NZ_AP022575:c2328237-2328022 [Mycobacterium shinjukuense]
ATGATCGACATCGATGACGACCTGCTTGCTCGGGCCGCCAAGGAACTCGGTACCACGACAAAGAAAGACACCGTGCATGC
CGCCCTAAGGGCCGCGCTGCGAGCCAGCGCCGCCCGCTCGCTCATGAACCGGATGGCCGAGAACGCAACCGGAATCGAGG
ACGAAGCCCTGGTCGACACAATGTGGCGAGACGGCCACCCAGCGAACACGGCATGA