Detailed information of TA system TA036197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2107629..2108239
• Replicon: chromosome
• Accession: NZ_AP022575
• Organism: Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N20_RS09320
• Protein ID: WP_083048400.1
• Coordinates: 2107629..2108021 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N20_RS09325
• Protein ID: WP_083048399.1
• Coordinates: 2108018..2108239 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N20_RS09270	2103483..2104145	+	663	WP_083048485.1	hypothetical protein	-
G6N20_RS09275	2104142..2104222	+	81	Protein_1834	type II toxin-antitoxin system VapB family antitoxin	-
G6N20_RS09280	2104308..2104445	+	138	WP_158084757.1	hypothetical protein	-
G6N20_RS09285	2104442..2105101	+	660	WP_083048406.1	hypothetical protein	-
G6N20_RS09290	2105098..2105760	+	663	WP_083048482.1	hypothetical protein	-
G6N20_RS09295	2105921..2106133	+	213	WP_083048404.1	type II toxin-antitoxin system VapB family antitoxin	-
G6N20_RS09300	2106133..2106546	+	414	WP_083048481.1	PIN domain nuclease	-
G6N20_RS09305	2106585..2106842	+	258	WP_083048479.1	ribbon-helix-helix domain-containing protein	-
G6N20_RS09310	2106839..2107216	+	378	WP_083048403.1	type II toxin-antitoxin system VapC family toxin	-
G6N20_RS09315	2107232..2107609	-	378	WP_083048401.1	hypothetical protein	-
G6N20_RS09320	2107629..2108021	-	393	WP_083048400.1	PIN domain-containing protein	Toxin
G6N20_RS09325	2108018..2108239	-	222	WP_083048399.1	type II toxin-antitoxin system antitoxin VapB20	Antitoxin
G6N20_RS09330	2109185..2109997	-	813	WP_163662916.1	ExeA family protein	-
G6N20_RS09335	2109994..2111401	-	1408	Protein_1846	DDE-type integrase/transposase/recombinase	-
G6N20_RS09340	2111456..2112082	-	627	WP_083052646.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2107629..2126575	18946

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-125)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-41)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14538.46 Da
• Isoelectric Point: 8.1098

>T36197 WP_083048400.1 NZ_AP022575:c2108021-2107629 [Mycobacterium shinjukuense]
MIFADTSFWAALGNVGDARHGAAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRFSTVVRVEHVTADLEE
QAWEWLARHDEREYSFVDATSFALMRKKSIRNAYAFDGDFSAAGFVEVRP

Nucleotide

• Length: 393 bp

>T36197 NZ_AP022575:c2108021-2107629 [Mycobacterium shinjukuense]
ATGATCTTCGCCGACACGTCCTTCTGGGCCGCACTTGGCAACGTCGGCGACGCTCGACACGGCGCCGCGAAGAGATTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCGAATCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCCGCGGTTGCCGCCGCCGCCATCCGCTTCAGCACCGTCGTTCGCGTCGAGCACGTCACGGCCGACCTGGAAGAG
CAGGCCTGGGAGTGGCTCGCACGGCACGACGAGCGAGAGTACTCGTTCGTCGACGCGACGAGCTTCGCGCTGATGCGCAA
GAAGAGCATCCGCAACGCGTATGCCTTCGACGGTGACTTCAGCGCTGCCGGATTTGTCGAGGTAAGACCGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8130.09 Da
• Isoelectric Point: 5.0900

>AT36197 WP_083048399.1 NZ_AP022575:c2108239-2108018 [Mycobacterium shinjukuense]
VKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFDGGADLSASVDDVVYGKRE

Nucleotide

• Length: 222 bp

>AT36197 NZ_AP022575:c2108239-2108018 [Mycobacterium shinjukuense]
GTGAAAAGGCTGCAGATCTACATCGACGAGGACGTCGACCGAGCGCTTGCCGTTGAAGCACGACGGCGGCGGACGTCGAA
GGCCGCGCTCATCCGGGAGTACGTCGCTGAACACCTCCGGCAGCCGGGCCCAGATCCGGTCGATGCCTTCGTCGGATCGT
TCGACGGGGGAGCCGACTTGTCCGCGTCCGTTGACGATGTGGTTTACGGAAAGCGCGAATGA