Detailed information of TA system TA036194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2105921..2106546
• Replicon: chromosome
• Accession: NZ_AP022575
• Organism: Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N20_RS09300
• Protein ID: WP_083048481.1
• Coordinates: 2106133..2106546 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N20_RS09295
• Protein ID: WP_083048404.1
• Coordinates: 2105921..2106133 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N20_RS09260	2101595..2101909	+	315	WP_083048408.1	hypothetical protein	-
G6N20_RS09265	2102206..2103353	+	1148	Protein_1832	alpha/beta hydrolase family protein	-
G6N20_RS09270	2103483..2104145	+	663	WP_083048485.1	hypothetical protein	-
G6N20_RS09275	2104142..2104222	+	81	Protein_1834	type II toxin-antitoxin system VapB family antitoxin	-
G6N20_RS09280	2104308..2104445	+	138	WP_158084757.1	hypothetical protein	-
G6N20_RS09285	2104442..2105101	+	660	WP_083048406.1	hypothetical protein	-
G6N20_RS09290	2105098..2105760	+	663	WP_083048482.1	hypothetical protein	-
G6N20_RS09295	2105921..2106133	+	213	WP_083048404.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
G6N20_RS09300	2106133..2106546	+	414	WP_083048481.1	PIN domain nuclease	Toxin
G6N20_RS09305	2106585..2106842	+	258	WP_083048479.1	ribbon-helix-helix domain-containing protein	-
G6N20_RS09310	2106839..2107216	+	378	WP_083048403.1	type II toxin-antitoxin system VapC family toxin	-
G6N20_RS09315	2107232..2107609	-	378	WP_083048401.1	hypothetical protein	-
G6N20_RS09320	2107629..2108021	-	393	WP_083048400.1	PIN domain-containing protein	-
G6N20_RS09325	2108018..2108239	-	222	WP_083048399.1	type II toxin-antitoxin system antitoxin VapB20	-
G6N20_RS09330	2109185..2109997	-	813	WP_163662916.1	ExeA family protein	-
G6N20_RS09335	2109994..2111401	-	1408	Protein_1846	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (4-42)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15060.06 Da
• Isoelectric Point: 5.4771

>T36194 WP_083048481.1 NZ_AP022575:2106133-2106546 [Mycobacterium shinjukuense]
MLFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSAKSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTVVWHYDEDYDRVAAITGQPTEWIVPRGTL

Nucleotide

• Length: 414 bp

>T36194 NZ_AP022575:2106133-2106546 [Mycobacterium shinjukuense]
ATGTTGTTTTGCGTCGACACCAGCGCGTGGCATCATGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAAGTCCGCCACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTGGTCATCGCCGCGGCGGCCGAACTCTCGGG
CACCGTCGTGTGGCATTACGACGAGGACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GGGGGACCCTTTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7445.53 Da
• Isoelectric Point: 12.0838

>AT36194 WP_083048404.1 NZ_AP022575:2105921-2106133 [Mycobacterium shinjukuense]
MSRRTTIDIDDILLARAQAALGTTGLKDTVDAALRAAVRQSARTRLTARIASGAGIDRSEALLAQTRPVR

Nucleotide

• Length: 213 bp

>AT36194 NZ_AP022575:2105921-2106133 [Mycobacterium shinjukuense]
ATGTCTCGGCGTACAACGATCGATATCGATGACATACTGCTGGCCCGTGCGCAAGCGGCGCTTGGTACCACCGGGTTGAA
GGACACGGTCGATGCCGCTTTGCGAGCCGCGGTGCGCCAGTCGGCGCGCACCCGGCTCACCGCGCGAATCGCCTCGGGTG
CCGGCATCGATCGGTCCGAGGCGCTGCTTGCCCAGACGCGTCCCGTGCGGTGA