Detailed information of TA system TA036193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2088808..2089448
• Replicon: chromosome
• Accession: NZ_AP022575
• Organism: Mycobacterium shinjukuense strain JCM 14233

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: G6N20_RS09195
• Protein ID: WP_003412970.1
• Coordinates: 2088808..2089227 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: G6N20_RS09200
• Protein ID: WP_003412975.1
• Coordinates: 2089224..2089448 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N20_RS09160	2084092..2084532	-	441	WP_083048439.1	PIN domain-containing protein	-
G6N20_RS09165	2084536..2085402	-	867	WP_083048437.1	hypothetical protein	-
G6N20_RS09170	2085586..2085813	+	228	WP_083048435.1	type II toxin-antitoxin system VapB family antitoxin	-
G6N20_RS09175	2085810..2086211	+	402	WP_083048490.1	PIN domain-containing protein	-
G6N20_RS09180	2086246..2087166	-	921	WP_083048433.1	restriction endonuclease	-
G6N20_RS09185	2087554..2087798	-	245	Protein_1816	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
G6N20_RS09190	2087857..2088807	+	951	WP_083048431.1	Lsr2 family protein	-
G6N20_RS09195	2088808..2089227	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
G6N20_RS09200	2089224..2089448	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
G6N20_RS09205	2089479..2092328	-	2850	WP_083048429.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
G6N20_RS09210	2092475..2092882	-	408	WP_083048427.1	antitermination protein NusB	-
G6N20_RS09215	2092879..2093373	-	495	WP_083048425.1	transcription antitermination factor NusB	-
G6N20_RS09220	2093376..2093939	-	564	WP_083048423.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T36193 WP_003412970.1 NZ_AP022575:c2089227-2088808 [Mycobacterium shinjukuense]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T36193 NZ_AP022575:c2089227-2088808 [Mycobacterium shinjukuense]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT36193 WP_003412975.1 NZ_AP022575:c2089448-2089224 [Mycobacterium shinjukuense]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT36193 NZ_AP022575:c2089448-2089224 [Mycobacterium shinjukuense]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09