Detailed information of TA system TA036180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 948626..949630
• Replicon: chromosome
• Accession: NZ_AP022573
• Organism: Mycobacterium saskatchewanense strain JCM 13016

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: G6N56_RS04360
• Protein ID: WP_085257274.1
• Coordinates: 948626..949204 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: G6N56_RS04365
• Protein ID: WP_085257275.1
• Coordinates: 949217..949630 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N56_RS04345	946111..946992	+	882	WP_085257273.1	CoA ester lyase	-
G6N56_RS04350	947154..947743	+	590	Protein_869	FAD-dependent monooxygenase	-
G6N56_RS04355	947799..948623	+	825	WP_085257392.1	FAD-dependent monooxygenase	-
G6N56_RS04360	948626..949204	-	579	WP_085257274.1	zeta toxin family protein	Toxin
G6N56_RS04365	949217..949630	-	414	WP_085257275.1	hypothetical protein	Antitoxin
G6N56_RS04370	949675..949937	-	263	Protein_873	DUF3253 domain-containing protein	-
G6N56_RS04375	949998..951431	-	1434	WP_158090744.1	MFS transporter	-
G6N56_RS04380	951654..952397	+	744	WP_085257277.1	GntR family transcriptional regulator	-
G6N56_RS04385	952411..953115	+	705	WP_085257278.1	phosphonatase-like hydrolase	-
G6N56_RS04390	953163..954155	+	993	WP_085257393.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (6-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (7-107)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21039.24 Da
• Isoelectric Point: 10.5018

>T36180 WP_085257274.1 NZ_AP022573:c949204-948626 [Mycobacterium saskatchewanense]
MNRLDLVIGPNGAGKSTFVEFTLAPLLPGSAFVNADEIAKRRWPAHPTAHAYEAARVAAETRSRLIELRRSFIAETVFSH
PSKLELIGTARAAGYTVVLHVLLIPEELAVQRVRHRVVAGGHDVPEDKIRQRFQRLWALAVAAIPRCDSATVYDNSALKG
PRIVAQLSGGELIGATSWPVWAPRELSARWPA

Nucleotide

• Length: 579 bp

>T36180 NZ_AP022573:c949204-948626 [Mycobacterium saskatchewanense]
ATGAACCGGCTGGACTTGGTCATCGGGCCCAACGGAGCCGGAAAATCCACCTTCGTCGAATTCACGCTCGCGCCCTTACT
GCCGGGAAGCGCGTTCGTCAACGCCGACGAGATCGCCAAGCGTCGCTGGCCCGCCCATCCGACCGCGCACGCCTACGAGG
CCGCCCGGGTGGCCGCCGAAACCCGTTCCCGGCTGATCGAATTGCGCCGCTCCTTCATCGCCGAAACCGTGTTCTCCCAT
CCCTCCAAGCTCGAGTTGATCGGCACCGCGCGCGCGGCCGGCTACACCGTGGTGTTGCACGTTCTGCTCATCCCCGAAGA
GCTGGCCGTGCAGCGGGTCAGACACCGAGTCGTCGCCGGCGGACATGACGTCCCCGAGGACAAGATCCGCCAACGGTTCC
AACGTCTTTGGGCGCTGGCCGTCGCCGCGATCCCCCGCTGCGACTCGGCCACCGTGTACGACAACAGCGCGCTGAAAGGG
CCTCGCATCGTCGCCCAGCTCAGTGGCGGCGAATTGATCGGCGCCACAAGTTGGCCGGTGTGGGCACCGCGCGAGTTGTC
AGCACGGTGGCCGGCGTGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 14768.54 Da
• Isoelectric Point: 7.3221

>AT36180 WP_085257275.1 NZ_AP022573:c949630-949217 [Mycobacterium saskatchewanense]
MVDVAADRVTRFAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSSQHTAARRRVEAALAGDLELRELSVEEGVVFNAE
IAAAVEENLVKAHYGDRLAQRGIATVALDEAGEIVEYPPDGRPVVVTAGRRRARRGK

Nucleotide

• Length: 414 bp

>AT36180 NZ_AP022573:c949630-949217 [Mycobacterium saskatchewanense]
ATGGTTGACGTTGCCGCGGATCGGGTAACCCGCTTCGCTGCCGATCTGGTGGACAGCGCAGCCGCCGAGGGGGCCCGACA
GAGCCGCTCGGCCAAGCAGCAACTCGACCATTGGGCGCGCGTGGGTCGGGCGGTCTCCAGCCAGCACACGGCCGCGCGCC
GGCGGGTCGAGGCCGCGCTGGCCGGCGACCTCGAGCTACGTGAACTCAGCGTCGAAGAGGGCGTCGTCTTCAACGCCGAG
ATCGCCGCGGCGGTCGAGGAAAACCTCGTCAAAGCCCACTACGGCGACCGGCTCGCCCAGCGCGGCATCGCCACCGTGGC
GCTCGACGAGGCCGGTGAGATCGTCGAATACCCGCCCGACGGACGCCCCGTCGTCGTGACCGCCGGCCGCCGGCGCGCCC
GCCGCGGAAAGTAA