Detailed information of TA system TA036179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 4230002..4230537
• Replicon: chromosome
• Accession: NZ_AP022572
• Organism: Mycobacterium shottsii strain JCM 12657

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G6N41_RS19615
• Protein ID: WP_198965432.1
• Coordinates: 4230205..4230537 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7I7LGS6
• Locus tag: G6N41_RS19610
• Protein ID: WP_036455700.1
• Coordinates: 4230002..4230208 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N41_RS19590	4225459..4226136	+	678	Protein_3882	ParA family protein	-
G6N41_RS19595	4226229..4227056	-	828	WP_020729183.1	DUF1906 domain-containing protein	-
G6N41_RS19600	4227384..4229070	-	1687	Protein_3884	GMC family oxidoreductase N-terminal domain-containing protein	-
G6N41_RS19605	4229319..4229669	-	351	WP_198965431.1	cupin domain-containing protein	-
G6N41_RS19610	4230002..4230208	+	207	WP_036455700.1	DUF3018 family protein	Antitoxin
G6N41_RS19615	4230205..4230537	+	333	WP_198965432.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N41_RS19620	4231144..4231485	+	342	WP_198967932.1	DUF2384 domain-containing protein	-
G6N41_RS19625	4231486..4232040	+	555	WP_020729188.1	RES domain-containing protein	-
G6N41_RS19630	4232318..4232551	+	234	WP_020729189.1	hypothetical protein	-
G6N41_RS19635	4232541..4232852	+	312	WP_020729190.1	type II toxin-antitoxin system PemK/MazF family toxin	-
G6N41_RS19640	4233105..4233830	-	726	WP_198968070.1	hypothetical protein	-
G6N41_RS19645	4233829..4234901	+	1073	Protein_3893	IS3 family transposase	-
G6N41_RS19650	4234970..4235329	-	360	WP_198965174.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11899.70 Da
• Isoelectric Point: 6.7245

>T36179 WP_198965432.1 NZ_AP022572:4230205-4230537 [Mycobacterium shottsii]
VNRGEIWTVAGDVYATKPHPAVIVQDDLFDATSSVTVAPMSSTLLDAPLMRIRIAGGDGRLSGLDHNSDVMIDKLTTVKR
SNVHARVGRLTAEQVVEVERVMMAFLGLAR

Nucleotide

• Length: 333 bp

>T36179 NZ_AP022572:4230205-4230537 [Mycobacterium shottsii]
GTGAATAGAGGGGAGATCTGGACCGTAGCGGGGGACGTCTACGCGACCAAACCGCATCCCGCGGTGATTGTTCAGGATGA
TCTCTTTGATGCCACGAGTTCGGTGACGGTTGCTCCGATGTCCAGCACCCTGTTGGATGCGCCGTTGATGCGCATCCGAA
TAGCCGGCGGAGACGGCCGGTTATCCGGACTTGATCACAACAGTGACGTGATGATCGACAAGCTCACAACCGTGAAAAGG
TCGAACGTCCATGCCCGAGTCGGTCGGCTGACAGCGGAGCAAGTCGTCGAGGTCGAGCGAGTGATGATGGCATTCCTCGG
CCTTGCGCGATAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7999.86 Da
• Isoelectric Point: 4.8359

>AT36179 WP_036455700.1 NZ_AP022572:4230002-4230208 [Mycobacterium shottsii]
MAVRDRVGEYRRRMRERGLRPLQVWVPDVRTESFAAEAHRQASLVARADERGDDQDFIEAISTPWDEE

Nucleotide

• Length: 207 bp

>AT36179 NZ_AP022572:4230002-4230208 [Mycobacterium shottsii]
ATGGCGGTTAGGGACAGGGTCGGCGAGTACCGACGGCGGATGCGGGAGCGGGGCCTGCGGCCGCTGCAGGTCTGGGTGCC
TGACGTGCGCACTGAGAGTTTTGCTGCCGAGGCGCATCGTCAGGCTTCGTTGGTCGCACGAGCGGACGAACGCGGCGATG
ATCAGGACTTCATTGAGGCCATCTCGACGCCATGGGATGAGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7LGS6