Detailed information of TA system TA036178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2142159..2142798
• Replicon: chromosome
• Accession: NZ_AP022572
• Organism: Mycobacterium shottsii strain JCM 12657

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: G6N41_RS10150
• Protein ID: WP_198967264.1
• Coordinates: 2142352..2142798 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: B2HEJ8
• Locus tag: G6N41_RS10145
• Protein ID: WP_011738542.1
• Coordinates: 2142159..2142347 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N41_RS10125	2137769..2138650	+	882	Protein_2001	MFS transporter	-
G6N41_RS10130	2138732..2139845	+	1114	Protein_2002	IS3 family transposase	-
G6N41_RS10135	2139842..2140276	+	435	WP_198967262.1	pentapeptide repeat-containing protein	-
G6N41_RS10140	2140234..2142057	+	1824	WP_198967263.1	PPE family protein	-
G6N41_RS10145	2142159..2142347	+	189	WP_011738542.1	antitoxin	Antitoxin
G6N41_RS10150	2142352..2142798	+	447	WP_198967264.1	SRPBCC family protein	Toxin
G6N41_RS10155	2142910..2144160	-	1251	WP_198967811.1	hypothetical protein	-
G6N41_RS10160	2144329..2145102	+	774	WP_198967265.1	VOC family protein	-
G6N41_RS10165	2145173..2145607	+	435	WP_012396159.1	hypothetical protein	-
G6N41_RS10170	2145613..2146086	-	474	WP_198967266.1	VOC family protein	-
G6N41_RS10175	2146103..2147623	-	1521	WP_198967267.1	carotenoid oxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15841.35 Da
• Isoelectric Point: 8.6574

>T36178 WP_198967264.1 NZ_AP022572:2142352-2142798 [Mycobacterium shottsii]
MAKLSGSIDVPLSPDEAWMHASDLSRFKEWLTIHRVWRSTLSETLEKGAVVESIVQVKGMHNRIKWTIVRYQPPEGMTLN
GDGVGGVKVKLMAKVAAGEEGSVVSFDVHLGGPALFGPIGMVVAAALRTDINASLRNFVTVFAPSAAG

Nucleotide

• Length: 447 bp

>T36178 NZ_AP022572:2142352-2142798 [Mycobacterium shottsii]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGTCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGCGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAAGAAGGCTCGGTCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGTATGGTCGTCGCCGCGGCGCTGCGCACCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGTCGGCCGCCGGCTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6586.35 Da
• Isoelectric Point: 4.7205

>AT36178 WP_011738542.1 NZ_AP022572:2142159-2142347 [Mycobacterium shottsii]
MGILDKAKDLLAQNADKVETVIDKAGEFVDEKTEGKYSDTIHKVQEGAKKVAGPGAAGEQQN

Nucleotide

• Length: 189 bp

>AT36178 NZ_AP022572:2142159-2142347 [Mycobacterium shottsii]
ATGGGAATCCTGGACAAGGCGAAAGACCTGCTGGCGCAGAACGCCGACAAGGTCGAGACGGTCATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAGGGGAAGTACTCCGACACCATCCACAAGGTGCAAGAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7LB02