Detailed information of TA system TA036176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 31779..32407
• Replicon: plasmid pJCM12603
• Accession: NZ_AP022571
• Organism: Mycolicibacterium poriferae strain JCM 12603

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N39_RS27180
• Protein ID: WP_163681009.1
• Coordinates: 31779..32189 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0J6YPF1
• Locus tag: G6N39_RS27185
• Protein ID: WP_048471216.1
• Coordinates: 32180..32407 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N39_RS27165	27232..29109	+	1878	WP_163681000.1	hypothetical protein	-
G6N39_RS27170	29189..30175	+	987	WP_163681002.1	non-homologous end-joining DNA ligase	-
G6N39_RS27175	30176..31732	-	1557	WP_163681005.1	serine/threonine protein kinase	-
G6N39_RS27180	31779..32189	-	411	WP_163681009.1	PIN domain nuclease	Toxin
G6N39_RS27185	32180..32407	-	228	WP_048471216.1	hypothetical protein	Antitoxin
G6N39_RS27190	32552..33508	+	957	WP_048424157.1	DUF4226 domain-containing protein	-
G6N39_RS27195	33540..33803	-	264	WP_163681011.1	hypothetical protein	-
G6N39_RS27200	33934..34266	+	333	WP_084032489.1	hypothetical protein	-
G6N39_RS27205	34345..35049	-	705	WP_163681014.1	hypothetical protein	-
G6N39_RS27210	35506..36705	+	1200	WP_163681017.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..216341	216341

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14666.85 Da
• Isoelectric Point: 4.8232

>T36176 WP_163681009.1 NZ_AP022571:c32189-31779 [Mycolicibacterium poriferae]
VAVTNWLIDKSAYTQLVASPDAQAWVERIERGLVRMSTVTRLQIGYSFRNGSQARSESGAPPLALMPLEYLTPAAEDRAV
GVQLQLADRGLHRAPAIPDLLVAAVAEMAGLTVLAVDTDFDLIAQVTGQAVERLRL

Nucleotide

• Length: 411 bp

>T36176 NZ_AP022571:c32189-31779 [Mycolicibacterium poriferae]
GTGGCGGTGACCAACTGGCTCATCGACAAATCGGCCTACACGCAACTGGTGGCCTCCCCAGACGCCCAGGCCTGGGTCGA
GCGCATCGAACGCGGCCTCGTGCGCATGAGCACCGTCACCCGCCTACAGATCGGCTACTCGTTCCGGAACGGATCGCAGG
CCCGCAGCGAATCAGGCGCACCGCCGCTGGCATTGATGCCGCTCGAGTACCTGACCCCAGCCGCCGAGGACCGCGCCGTG
GGCGTGCAGCTCCAGCTGGCCGACCGTGGGCTGCACCGCGCCCCGGCGATCCCGGATCTGCTTGTGGCCGCGGTCGCCGA
GATGGCCGGCCTTACCGTGCTTGCCGTCGACACGGACTTCGACCTGATCGCGCAGGTCACCGGACAAGCCGTCGAACGGC
TCCGCCTATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8124.04 Da
• Isoelectric Point: 4.3664

>AT36176 WP_048471216.1 NZ_AP022571:c32407-32180 [Mycolicibacterium poriferae]
MGDVLIRGLSDAAIARIDADAAARGLSRQEYLRTRFETEGSVSAPTRTMTVDDLRRAAAAATDLDDPDIMDAAWR

Nucleotide

• Length: 228 bp

>AT36176 NZ_AP022571:c32407-32180 [Mycolicibacterium poriferae]
ATGGGCGATGTGCTGATCCGCGGACTGTCCGACGCGGCGATCGCGCGTATCGACGCCGACGCAGCCGCCCGCGGTCTCTC
GCGCCAAGAATATCTGCGAACCAGATTCGAGACCGAAGGTTCAGTGAGCGCCCCCACGCGGACCATGACCGTCGACGATC
TACGCCGCGCCGCCGCGGCGGCCACCGACCTCGACGACCCCGACATCATGGACGCCGCGTGGCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0J6YPF1