Detailed information of TA system TA036166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4328256..4328848
• Replicon: chromosome
• Accession: NZ_AP022569
• Organism: Mycobacterium cookii strain JCM 12404

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N27_RS20345
• Protein ID: WP_163779540.1
• Coordinates: 4328450..4328848 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N27_RS20340
• Protein ID: WP_163779538.1
• Coordinates: 4328256..4328453 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N27_RS20325	4324095..4325387	-	1293	WP_163779532.1	DEAD/DEAH box helicase	-
G6N27_RS20330	4325779..4326720	+	942	WP_163779534.1	class I SAM-dependent methyltransferase	-
G6N27_RS20335	4326721..4328178	-	1458	WP_163779536.1	DUF1254 domain-containing protein	-
G6N27_RS20340	4328256..4328453	+	198	WP_163779538.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
G6N27_RS20345	4328450..4328848	+	399	WP_163779540.1	PIN domain nuclease	Toxin
G6N27_RS20350	4328919..4330484	-	1566	WP_163779542.1	serine/threonine protein kinase	-
G6N27_RS20355	4330625..4333267	+	2643	WP_163779544.1	GH92 family glycosyl hydrolase	-
G6N27_RS20360	4333295..4333639	-	345	WP_179963313.1	hemophore-related protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-41)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14698.21 Da
• Isoelectric Point: 7.0844

>T36166 WP_163779540.1 NZ_AP022569:4328450-4328848 [Mycobacterium cookii]
VIVVDTSVWIDVLNDTPAPQATRCVELIEAGQPVALTDVILTEILQGLRSDREAMRVERHLRAFPILRLAELDDFVLAAK
LYRAARRAGVTIRKTLDCLIAAPCVRTGVPLLHADADFDRLATCTPLRIWTR

Nucleotide

• Length: 399 bp

>T36166 NZ_AP022569:4328450-4328848 [Mycobacterium cookii]
GTGATTGTCGTCGACACCAGTGTCTGGATCGACGTCCTGAACGACACTCCCGCGCCTCAGGCGACGCGTTGTGTGGAGCT
AATTGAAGCCGGACAGCCAGTTGCGTTAACCGACGTGATCTTGACCGAGATCCTGCAGGGGCTGCGGTCGGATCGGGAGG
CGATGCGGGTGGAGCGGCATCTACGCGCCTTCCCAATCCTTCGTTTGGCGGAGCTCGACGATTTTGTGCTGGCGGCCAAG
CTGTATCGCGCGGCGCGACGTGCTGGTGTAACCATCCGGAAGACTCTCGACTGTCTGATCGCAGCTCCCTGTGTGCGTAC
CGGTGTTCCGCTGTTGCACGCGGACGCAGACTTTGACCGCCTCGCCACCTGCACACCGCTTCGGATCTGGACGCGCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7497.57 Da
• Isoelectric Point: 11.3253

>AT36166 WP_163779538.1 NZ_AP022569:4328256-4328453 [Mycobacterium cookii]
MRTNIEIDDNVLREAQRLLGTKTKRDTVDLALRELVARHRQVGILQLRGKVRWEGDLAESRSGRS

Nucleotide

• Length: 198 bp

>AT36166 NZ_AP022569:4328256-4328453 [Mycobacterium cookii]
ATGCGCACCAATATTGAGATCGACGACAACGTCCTGCGCGAGGCTCAGCGCTTGCTCGGTACGAAAACTAAGCGTGACAC
CGTCGATCTGGCACTGCGCGAACTCGTTGCCCGGCATCGCCAGGTCGGGATCCTGCAACTGCGCGGAAAGGTGCGTTGGG
AAGGCGACTTGGCCGAGAGCCGTAGCGGCCGGTCGTGA