Detailed information of TA system TA036163

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/ParD(antitoxin)
• Location: 5261380..5262370
• Replicon: chromosome
• Accession: NZ_AP022568
• Organism: Mycobacterium simiae strain JCM 12377

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: G6N33_RS24475
• Protein ID: WP_101528579.1
• Coordinates: 5261792..5262370 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: A0A7I7KNM5
• Locus tag: G6N33_RS24470
• Protein ID: WP_044506174.1
• Coordinates: 5261380..5261769 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N33_RS24455	5257109..5259187	+	2079	WP_044506176.1	protein kinase	-
G6N33_RS24460	5259738..5260877	+	1140	WP_049918926.1	cytochrome P450	-
G6N33_RS24465	5260928..5261308	-	381	WP_049918925.1	hypothetical protein	-
G6N33_RS24470	5261380..5261769	+	390	WP_044506174.1	hypothetical protein	Antitoxin
G6N33_RS24475	5261792..5262370	+	579	WP_101528579.1	zeta toxin family protein	Toxin
G6N33_RS24480	5262561..5262733	-	173	Protein_4838	helix-turn-helix transcriptional regulator	-
G6N33_RS24485	5263060..5263245	+	186	WP_044511842.1	hypothetical protein	-
G6N33_RS24490	5263646..5264482	+	837	WP_101528578.1	hypothetical protein	-
G6N33_RS24495	5264642..5266201	-	1560	WP_044511838.1	GMC family oxidoreductase N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5236303..5270334	34031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-138)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21225.31 Da
• Isoelectric Point: 9.5573

>T36163 WP_101528579.1 NZ_AP022568:5261792-5262370 [Mycobacterium simiae]
MKRLDLVVGPNGAGKSTFIAFTLAPLLPRSVFVNADEIAKRRWPDDPAGHAYDAARIAADTRAKLIEAGVSFIAETVFSH
PSKLDLIRSAHDASYVIALHVLLIPEGLAVERVRRRVGHGGHDVPEGKIRERHRRLWHLVAEAIEIADTATVYDNSRLDG
PRIVEQLTAGTVIGAPTWPAWASDELLRRWPR

Nucleotide

• Length: 579 bp

>T36163 NZ_AP022568:5261792-5262370 [Mycobacterium simiae]
ATGAAGCGGCTGGACCTCGTCGTCGGCCCGAACGGCGCTGGTAAGTCGACTTTCATCGCGTTTACCTTGGCGCCGTTGTT
GCCACGCAGCGTATTCGTCAATGCCGACGAGATCGCAAAGCGGCGTTGGCCCGATGATCCCGCCGGGCATGCCTACGATG
CAGCCCGGATCGCTGCAGATACCCGCGCCAAGCTGATCGAGGCTGGCGTGTCGTTCATCGCCGAGACCGTGTTCTCCCAT
CCCTCGAAGCTGGACCTGATCCGCTCAGCCCACGATGCGAGCTATGTCATCGCTTTGCACGTGTTGCTGATCCCCGAGGG
GCTAGCCGTCGAACGGGTGCGACGACGCGTCGGTCACGGCGGCCACGACGTCCCCGAGGGCAAGATCCGCGAACGGCACC
GCCGATTGTGGCATTTGGTGGCCGAAGCGATCGAGATCGCCGACACTGCAACGGTCTACGATAATTCACGCCTCGACGGT
CCCCGGATCGTCGAGCAACTGACTGCGGGCACGGTCATTGGCGCACCGACGTGGCCAGCGTGGGCGTCCGACGAGCTACT
CCGACGCTGGCCCCGGTAG

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13740.06 Da
• Isoelectric Point: 4.5152

>AT36163 WP_044506174.1 NZ_AP022568:5261380-5261769 [Mycobacterium simiae]
MTNVADRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSSHQSASRRRVEAALAGDLDTSRLSEEEGLVFNAEI
SAAIEESLATAHYGDLLSMRGITTVALNDDGQIVEYRPDGTTSVVDVEG

Nucleotide

• Length: 390 bp

>AT36163 NZ_AP022568:5261380-5261769 [Mycobacterium simiae]
ATGACGAACGTCGCCGATCGCGTCACCAGGGTCGCCGCCGACCTGGTGGACAGCGCTGCTGCCGAGGGCGCTCGCCAGAG
CCGGTCGGCCAAGCAGCAACTCGATCATTGGGCTCGCGTGGGCCGGGCGGTTTCGAGCCATCAGTCGGCCTCACGTCGCC
GCGTCGAGGCCGCGCTGGCAGGTGACCTGGACACGAGCCGTTTGTCCGAGGAGGAGGGGCTGGTCTTCAACGCCGAGATC
TCGGCCGCTATCGAGGAGTCCCTGGCCACCGCCCATTACGGTGATCTGCTGTCAATGCGCGGCATCACGACAGTGGCGCT
CAACGACGACGGCCAGATCGTCGAGTACAGGCCAGACGGCACTACCTCGGTGGTCGACGTCGAGGGATGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7KNM5