Detailed information of TA system TA036161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 6515734..6516730
• Replicon: chromosome
• Accession: NZ_AP022567
• Organism: Mycolicibacterium mageritense strain JCM 12375

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: X5L6H2
• Locus tag: G6N67_RS31375
• Protein ID: WP_036442085.1
• Coordinates: 6516152..6516730 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: X5LR27
• Locus tag: G6N67_RS31370
• Protein ID: WP_036442083.1
• Coordinates: 6515734..6516123 (+)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N67_RS31355	6511482..6512228	-	747	WP_036442353.1	alpha/beta hydrolase	-
G6N67_RS31360	6512443..6513744	+	1302	WP_036442078.1	TrpB-like pyridoxal phosphate-dependent enzyme	-
G6N67_RS31365	6513761..6515614	-	1854	WP_036442080.1	serine/threonine protein kinase	-
G6N67_RS31370	6515734..6516123	+	390	WP_036442083.1	hypothetical protein	Antitoxin
G6N67_RS31375	6516152..6516730	+	579	WP_036442085.1	zeta toxin family protein	Toxin
G6N67_RS31380	6516646..6518127	-	1482	WP_081812839.1	cytosine permease	-
G6N67_RS31385	6518442..6519971	+	1530	WP_051579294.1	biotin carboxylase	-
G6N67_RS31390	6520065..6521573	+	1509	WP_036442087.1	PucR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 20802.64 Da
• Isoelectric Point: 8.5195

>T36161 WP_036442085.1 NZ_AP022567:6516152-6516730 [Mycolicibacterium mageritense]
VKRLDLVVGSNGAGKSTFVELTLGPLLPNSPFVNADEIAKRRWPEDPATHSYEAARIAAQTRARLIERGDSFIAETVFSH
PSKLDLIDTANAAGYSVILHVVMIPEDLAVLRVRHRVEAGGHDVPEAKIRERYQRLWPLVAAAAARSESAAFYDNSTTKG
PRIVAQVTGGFVVGSPAWPNWTPAALVSTWPG

Nucleotide

• Length: 579 bp

>T36161 NZ_AP022567:6516152-6516730 [Mycolicibacterium mageritense]
GTGAAGCGGCTCGACCTCGTCGTCGGATCCAACGGCGCGGGCAAGTCGACGTTCGTCGAACTCACGCTCGGGCCCCTGTT
GCCCAACAGTCCTTTCGTCAACGCCGACGAGATCGCCAAGCGCCGCTGGCCCGAGGATCCCGCCACCCACTCTTATGAGG
CCGCACGTATCGCGGCGCAAACCCGGGCCAGGCTCATCGAGCGGGGCGATTCGTTCATCGCCGAAACCGTGTTCTCCCAC
CCGTCCAAACTGGACCTGATCGACACCGCGAACGCGGCCGGCTACAGCGTCATCCTGCACGTCGTGATGATCCCGGAGGA
TCTCGCGGTGCTGCGCGTGCGACACCGCGTCGAGGCAGGTGGTCACGATGTGCCCGAAGCGAAGATTCGTGAACGCTACC
AACGGCTCTGGCCGCTCGTCGCCGCCGCGGCGGCGCGCTCGGAATCGGCGGCGTTCTACGACAACAGCACCACTAAGGGC
CCCCGCATCGTGGCTCAGGTCACGGGCGGTTTCGTTGTGGGGTCACCGGCCTGGCCGAATTGGACACCGGCCGCGCTGGT
GTCGACGTGGCCAGGGTAG

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13830.32 Da
• Isoelectric Point: 4.9265

>AT36161 WP_036442083.1 NZ_AP022567:6515734-6516123 [Mycolicibacterium mageritense]
MTDTADRVTRFAADLMDSAAAEGARQSRSAKQQLDHWARVGRAVSSRQSAARRKVEAALAGDTPLRDLTIEEGVVFNAEI
SAAIEENLARADYGTTLAARGVTTVALDDNGEIVQYRPDGSTTVLVPQR

Nucleotide

• Length: 390 bp

>AT36161 NZ_AP022567:6515734-6516123 [Mycolicibacterium mageritense]
ATGACTGACACCGCAGACCGCGTGACTCGGTTCGCCGCTGATCTGATGGACAGCGCTGCCGCGGAAGGCGCGCGCCAGAG
CCGCTCGGCCAAGCAGCAGCTCGACCACTGGGCGCGTGTCGGACGGGCCGTGTCAAGCCGGCAGAGCGCCGCACGCCGCA
AGGTCGAGGCGGCCTTGGCGGGCGACACCCCGTTGCGTGATCTGACCATCGAGGAGGGCGTCGTGTTCAACGCCGAGATC
TCCGCGGCCATCGAGGAGAACCTGGCGCGCGCCGATTACGGCACGACACTGGCCGCCCGTGGGGTCACCACCGTCGCGCT
CGACGACAACGGCGAGATCGTGCAGTACCGCCCTGATGGCAGCACCACGGTGTTGGTGCCTCAGCGGTGA

Structures

Toxin

• AlphaFold DB: X5L6H2

Antitoxin

• AlphaFold DB: X5LR27