Detailed information of TA system TA036159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1077999..1078631
• Replicon: chromosome
• Accession: NZ_AP022567
• Organism: Mycolicibacterium mageritense strain JCM 12375

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: X5LHJ8
• Locus tag: G6N67_RS05210
• Protein ID: WP_036433725.1
• Coordinates: 1077999..1078448 (-)
• Length: 150 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X5LDU2
• Locus tag: G6N67_RS05215
• Protein ID: WP_036433724.1
• Coordinates: 1078452..1078631 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N67_RS05190	1073361..1074614	-	1254	WP_036435803.1	Tm-1-like ATP-binding domain-containing protein	-
G6N67_RS05195	1074632..1075441	-	810	WP_036433728.1	SDR family oxidoreductase	-
G6N67_RS05200	1075470..1076306	-	837	WP_036433727.1	phosphoenolpyruvate hydrolase family protein	-
G6N67_RS05205	1076678..1077904	+	1227	WP_081812656.1	MHS family MFS transporter	-
G6N67_RS05210	1077999..1078448	-	450	WP_036433725.1	SRPBCC family protein	Toxin
G6N67_RS05215	1078452..1078631	-	180	WP_036433724.1	antitoxin	Antitoxin
G6N67_RS05220	1078702..1081188	-	2487	WP_036433723.1	HAD-IC family P-type ATPase	-
G6N67_RS05225	1081217..1082800	-	1584	WP_051578807.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 150 a.a.
• Molecular weight: 16149.76 Da
• Isoelectric Point: 9.6575

>T36159 WP_036433725.1 NZ_AP022567:c1078448-1077999 [Mycolicibacterium mageritense]
MAKLSVSVDVPLPPEKAWEYASDLSRYDEWLSIHRAWRSKLPETLEKGTVIDSIVEVKGMLNRVKWTLVNYKPPHSLTLN
GDGRGGVKVKLIGKIKPAPVDGAEGATVTFDVHLGGPALFGPIGMVVASALKGDIQESLNKFKALYAPS

Nucleotide

• Length: 450 bp

>T36159 NZ_AP022567:c1078448-1077999 [Mycolicibacterium mageritense]
ATGGCAAAACTGTCAGTCTCCGTCGATGTGCCGTTGCCGCCGGAGAAGGCGTGGGAGTACGCCTCCGATCTGTCCCGTTA
CGACGAGTGGTTGAGCATCCACCGCGCCTGGCGGTCGAAGTTGCCGGAAACCCTTGAGAAGGGCACCGTCATCGACTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGGTCAAATGGACCCTCGTCAACTACAAGCCGCCGCATTCGTTGACCCTCAAC
GGCGACGGCCGCGGCGGGGTCAAAGTCAAGCTGATCGGAAAGATCAAGCCCGCGCCGGTCGACGGCGCCGAGGGCGCCAC
CGTGACGTTCGACGTCCATCTCGGCGGCCCGGCGCTGTTCGGTCCGATCGGCATGGTGGTGGCGTCCGCGCTCAAAGGCG
ACATCCAGGAGTCGCTCAACAAGTTCAAGGCGCTCTACGCACCGTCGTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6443.21 Da
• Isoelectric Point: 8.5694

>AT36159 WP_036433724.1 NZ_AP022567:c1078631-1078452 [Mycolicibacterium mageritense]
MGFLDKAKDLLSKNADKVDTAIDKAGDVLDQKTQGKYSQHVDKAQDAAKNAIRKEGPQQ

Nucleotide

• Length: 180 bp

>AT36159 NZ_AP022567:c1078631-1078452 [Mycolicibacterium mageritense]
ATGGGATTTTTGGACAAGGCGAAAGATCTGCTTTCCAAGAACGCCGACAAGGTGGACACGGCGATCGACAAGGCCGGTGA
CGTCCTCGATCAGAAGACGCAGGGCAAGTACAGCCAGCATGTCGACAAGGCGCAGGATGCCGCCAAGAACGCCATCCGTA
AAGAAGGGCCCCAGCAGTAA

Structures

Toxin

• AlphaFold DB: X5LHJ8

Antitoxin

• AlphaFold DB: X5LDU2