Detailed information of TA system TA036154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 803736..804271
• Replicon: chromosome
• Accession: NZ_AP022565
• Organism: Mycolicibacterium alvei strain JCM 12272

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G6N44_RS03830
• Protein ID: WP_163669557.1
• Coordinates: 803939..804271 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G6N44_RS03825
• Protein ID: WP_163661272.1
• Coordinates: 803736..803942 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N44_RS03805	798977..800662	-	1686	WP_163661266.1	GMC family oxidoreductase N-terminal domain-containing protein	-
G6N44_RS03810	800810..801978	+	1169	Protein_753	alpha/beta hydrolase	-
G6N44_RS03815	802310..802861	-	552	WP_163661268.1	YaeQ family protein	-
G6N44_RS03820	803097..803501	-	405	WP_163661270.1	PaaI family thioesterase	-
G6N44_RS03825	803736..803942	+	207	WP_163661272.1	antitoxin MazE family protein	Antitoxin
G6N44_RS03830	803939..804271	+	333	WP_163669557.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N44_RS03835	804325..804534	+	210	WP_163669559.1	hypothetical protein	-
G6N44_RS03840	804531..804863	+	333	WP_163661274.1	type II toxin-antitoxin system PemK/MazF family toxin	-
G6N44_RS03845	805025..805387	+	363	WP_163661276.1	metallopeptidase family protein	-
G6N44_RS03850	805655..806362	+	708	WP_163661278.1	hypothetical protein	-
G6N44_RS03855	806464..807090	+	627	WP_163661280.1	RES domain-containing protein	-
G6N44_RS03860	807388..808326	+	939	WP_163669561.1	alpha/beta hydrolase fold domain-containing protein	-
G6N44_RS03865	808499..808720	+	222	WP_083119727.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
G6N44_RS03870	808740..808862	+	123	Protein_765	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11915.79 Da
• Isoelectric Point: 7.2030

>T36154 WP_163669557.1 NZ_AP022565:803939-804271 [Mycolicibacterium alvei]
VNRGEIWTVVGGVYAAKPRPAVIVQDDLFDATSSVTLAPMTTTLLDAPLMRIRIGGGDGRLAGLDHDSDVMIDKLTTVRR
SNVHARVGRLTAEQLVEIERAMMAYLGLAR

Nucleotide

• Length: 333 bp

>T36154 NZ_AP022565:803939-804271 [Mycolicibacterium alvei]
GTGAATCGAGGGGAAATCTGGACCGTGGTCGGAGGTGTTTACGCGGCGAAGCCGCGTCCGGCGGTGATCGTTCAGGATGA
CCTTTTCGATGCCACGAGTTCGGTCACCCTAGCTCCGATGACCACCACGCTGTTAGATGCGCCGCTGATGCGCATCCGCA
TCGGCGGCGGAGACGGGCGACTTGCGGGACTCGACCACGATAGTGACGTGATGATCGACAAGCTCACGACGGTCAGAAGG
TCGAACGTCCATGCCCGGGTTGGCCGACTGACAGCCGAGCAACTGGTTGAGATCGAGCGGGCAATGATGGCATACCTCGG
CCTTGCTCGATGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7989.82 Da
• Isoelectric Point: 4.6412

>AT36154 WP_163661272.1 NZ_AP022565:803736-803942 [Mycolicibacterium alvei]
MAVRERVGEYRRRMRERGLRPLQVWVPDVRTETFAAEAHRQASLVAAADENSDDQDFIEAISTRWDEE

Nucleotide

• Length: 207 bp

>AT36154 NZ_AP022565:803736-803942 [Mycolicibacterium alvei]
ATGGCGGTGAGGGAACGAGTCGGTGAGTACCGACGACGGATGCGTGAGCGTGGCCTCCGGCCATTACAGGTCTGGGTGCC
TGATGTGCGGACCGAGACCTTCGCGGCCGAGGCCCACAGGCAGGCCTCGCTGGTCGCGGCAGCGGACGAAAACAGCGACG
ACCAAGACTTCATTGAGGCAATCTCGACGCGGTGGGACGAGGAGTGA