Detailed information of TA system TA036147

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2278200..2278764
• Replicon: chromosome
• Accession: NZ_AP022562
• Organism: Mycobacterium novum strain JCM 6391

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F5YTQ4
• Locus tag: G6N19_RS10430
• Protein ID: WP_013831006.1
• Coordinates: 2278200..2278547 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G6N19_RS10435
• Protein ID: WP_193464874.1
• Coordinates: 2278534..2278764 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N19_RS10410	2275879..2276283	-	405	WP_193464871.1	type II toxin-antitoxin system VapC family toxin	-
G6N19_RS10415	2276268..2276513	-	246	WP_193464872.1	hypothetical protein	-
G6N19_RS10420	2276617..2277030	-	414	WP_013831008.1	GtrA family protein	-
G6N19_RS10425	2277064..2278170	+	1107	WP_193464873.1	mandelate racemase/muconate lactonizing enzyme family protein	-
G6N19_RS10430	2278200..2278547	-	348	WP_013831006.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G6N19_RS10435	2278534..2278764	-	231	WP_193464874.1	ribbon-helix-helix domain-containing protein	Antitoxin
G6N19_RS10440	2278795..2279625	-	831	WP_162112958.1	ABC transporter permease	-
G6N19_RS10445	2279622..2280539	-	918	WP_193464875.1	glycosyltransferase family 2 protein	-
G6N19_RS10450	2280536..2281339	-	804	WP_083038002.1	ABC transporter ATP-binding protein	-
G6N19_RS10455	2281346..2281915	-	570	WP_193464876.1	bacterial proteasome activator family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-38)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12593.36 Da
• Isoelectric Point: 10.3987

>T36147 WP_013831006.1 NZ_AP022562:c2278547-2278200 [Mycobacterium novum]
MRRGDIWQVDFDPTRGSEANKYRPAVVVSNDRANATAGRLGRGVVTVVPVTSNTATVYPFQVLLPATSTGLAVDSKAQAE
QIRSVAVERLQRRIGRLPAIELTQLDEALRLHLDL

Nucleotide

• Length: 348 bp

>T36147 NZ_AP022562:c2278547-2278200 [Mycobacterium novum]
ATGCGCCGCGGTGACATCTGGCAGGTCGACTTCGACCCGACCCGGGGAAGCGAAGCGAACAAGTACCGCCCGGCCGTCGT
CGTCAGCAACGACCGGGCCAATGCAACGGCCGGCCGACTCGGTCGCGGCGTGGTGACCGTCGTCCCGGTGACCAGCAACA
CCGCTACTGTCTACCCGTTCCAGGTGCTGTTGCCGGCCACCTCCACCGGCCTGGCCGTCGATTCGAAAGCACAAGCCGAA
CAGATCAGGTCGGTTGCCGTCGAACGTCTGCAACGGCGAATCGGCCGCCTCCCGGCGATCGAACTCACCCAACTCGACGA
GGCGCTGCGACTGCACCTCGACTTGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8369.09 Da
• Isoelectric Point: 4.0424

>AT36147 WP_193464874.1 NZ_AP022562:c2278764-2278534 [Mycobacterium novum]
MKLSISLSAEDVEALDAYAQRTGLPSRSAAVQHAIRMLRYPTLEDDYADAWAQWSADGEDQVWDRATDDGAGDAPR

Nucleotide

• Length: 231 bp

>AT36147 NZ_AP022562:c2278764-2278534 [Mycobacterium novum]
ATGAAGTTGAGTATCAGTCTTTCCGCCGAGGATGTCGAGGCTCTGGACGCCTACGCGCAACGCACGGGTTTGCCGTCACG
TTCGGCGGCCGTCCAGCATGCGATTCGGATGCTGCGCTACCCCACCCTCGAAGACGACTACGCCGACGCTTGGGCGCAGT
GGTCGGCCGACGGTGAGGATCAGGTTTGGGACCGGGCCACCGACGACGGGGCCGGCGATGCGCCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7I9YEP4