Detailed information of TA system TA036143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1359474..1360087
• Replicon: chromosome
• Accession: NZ_AP022562
• Organism: Mycobacterium novum strain JCM 6391

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5Z2F7
• Locus tag: G6N19_RS06215
• Protein ID: WP_013827511.1
• Coordinates: 1359474..1359878 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G6N19_RS06220
• Protein ID: WP_013827510.1
• Coordinates: 1359875..1360087 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N19_RS06195	1354803..1355006	-	204	WP_193465993.1	hypothetical protein	-
G6N19_RS06200	1355094..1355729	+	636	WP_013827515.1	ankyrin repeat domain-containing protein	-
G6N19_RS06205	1355751..1356626	+	876	WP_013827514.1	SDR family NAD(P)-dependent oxidoreductase	-
G6N19_RS06210	1356983..1359349	+	2367	WP_193465994.1	flippase-like domain-containing protein	-
G6N19_RS06215	1359474..1359878	-	405	WP_013827511.1	PIN domain nuclease	Toxin
G6N19_RS06220	1359875..1360087	-	213	WP_013827510.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
G6N19_RS06225	1360111..1361190	-	1080	WP_193465995.1	galactokinase	-
G6N19_RS06230	1361187..1362272	-	1086	WP_193465996.1	galactose-1-phosphate uridylyltransferase	-
G6N19_RS06235	1362269..1362745	-	477	WP_013827507.1	DUF309 domain-containing protein	-
G6N19_RS06240	1362767..1363555	-	789	WP_013827506.1	class I SAM-dependent methyltransferase	-
G6N19_RS06245	1363671..1364914	-	1244	Protein_1219	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-43)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14652.70 Da
• Isoelectric Point: 5.1895

>T36143 WP_013827511.1 NZ_AP022562:c1359878-1359474 [Mycobacterium novum]
MILVDTSVWIEYFRATGSAAALEVRRLLSEEPDQVVTCEPIAMEILAGASNEQTYRMLERLVNGLSSLGVETATDFRSAA
GIYRAARSTGQTIRSINDCLIAAVALRHHARVAHRDADFEVIEGITGLMTTSLR

Nucleotide

• Length: 405 bp

>T36143 NZ_AP022562:c1359878-1359474 [Mycobacterium novum]
ATGATTCTGGTCGACACCTCGGTATGGATCGAATACTTCCGGGCAACCGGGTCGGCGGCAGCGCTGGAAGTGCGGCGGCT
GCTCTCCGAAGAACCCGATCAAGTAGTGACCTGCGAGCCGATCGCGATGGAGATCTTGGCTGGCGCAAGCAACGAGCAGA
CCTACCGAATGCTTGAGCGGCTGGTCAACGGGTTGTCATCGCTGGGGGTCGAGACGGCAACGGATTTTCGGTCGGCCGCG
GGCATCTACCGTGCTGCCAGAAGCACCGGCCAGACGATTCGCAGCATCAACGACTGCCTTATCGCCGCGGTCGCGCTGCG
CCATCATGCCCGGGTTGCGCACCGCGACGCCGACTTCGAGGTCATCGAGGGGATAACCGGCCTGATGACCACATCGCTGC
GCTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7682.79 Da
• Isoelectric Point: 4.4013

>AT36143 WP_013827510.1 NZ_AP022562:c1360087-1359875 [Mycobacterium novum]
MARTNIEIDDELTAEVMRRFGVTTKKAAVDLALRRLVGAPLTREFLLGLEGIGWAGDLDELRSEQPGELG

Nucleotide

• Length: 213 bp

>AT36143 NZ_AP022562:c1360087-1359875 [Mycobacterium novum]
ATGGCACGCACCAATATCGAAATCGATGACGAACTCACCGCTGAGGTGATGCGTCGCTTCGGCGTGACCACCAAGAAGGC
GGCTGTTGACCTCGCGTTACGCCGTCTCGTCGGCGCGCCCTTGACCCGGGAGTTTCTGCTGGGCCTCGAGGGCATCGGGT
GGGCGGGTGACCTCGATGAGTTGCGCAGCGAACAACCCGGCGAACTCGGATGA

Structures

Toxin

• AlphaFold DB: A0A7I7JL80