Detailed information of TA system TA036140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 78863..79503
• Replicon: chromosome
• Accession: NZ_AP022562
• Organism: Mycobacterium novum strain JCM 6391

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G6N19_RS00365
• Protein ID: WP_013828728.1
• Coordinates: 79114..79503 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F5Z1W7
• Locus tag: G6N19_RS00360
• Protein ID: WP_013828729.1
• Coordinates: 78863..79117 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N19_RS00320	74151..74594	+	444	WP_083038228.1	Zn-ribbon domain-containing OB-fold protein	-
G6N19_RS00325	74591..75793	+	1203	WP_083038134.1	lipid-transfer protein	-
G6N19_RS00330	75880..76311	-	432	WP_013828735.1	type II toxin-antitoxin system VapC family toxin	-
G6N19_RS00335	76318..76569	-	252	WP_013828734.1	hypothetical protein	-
G6N19_RS00340	76612..76848	+	237	WP_013828733.1	DUF167 domain-containing protein	-
G6N19_RS00345	76857..77603	-	747	WP_013828732.1	proteasome subunit alpha	-
G6N19_RS00350	77600..78484	-	885	WP_193465552.1	proteasome subunit beta	-
G6N19_RS00355	78481..78675	-	195	WP_013828730.1	ubiquitin-like protein Pup	-
G6N19_RS00360	78863..79117	+	255	WP_013828729.1	hypothetical protein	Antitoxin
G6N19_RS00365	79114..79503	+	390	WP_013828728.1	PIN domain-containing protein	Toxin
G6N19_RS00370	79558..81066	-	1509	WP_013828727.1	proteasome accessory factor PafA2	-
G6N19_RS00375	81097..81744	-	648	WP_081465647.1	DUF2293 domain-containing protein	-
G6N19_RS00380	81762..81929	-	168	WP_158019458.1	hypothetical protein	-
G6N19_RS00385	82023..82643	-	621	WP_193465553.1	TetR/AcrR family transcriptional regulator	-
G6N19_RS00390	82768..83187	+	420	WP_193465554.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (3-118)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13896.73 Da
• Isoelectric Point: 4.7729

>T36140 WP_013828728.1 NZ_AP022562:79114-79503 [Mycobacterium novum]
MIVVDAAALVDALTGVDGTDELRSELADEELHAPALLDFEVVSALRGLTRGGQLSAARAHDALADFDALAIRRWPGSAEL
RLRAFTLRDNLTAYDAAYVALAEALECAVLTRDRRLARSGGHSVEIRVR

Nucleotide

• Length: 390 bp

>T36140 NZ_AP022562:79114-79503 [Mycobacterium novum]
ATGATCGTCGTCGATGCCGCAGCGCTCGTCGACGCGCTCACCGGCGTCGACGGCACCGACGAACTGCGCAGTGAACTCGC
CGACGAGGAACTACATGCCCCGGCGTTGTTGGACTTCGAAGTCGTCTCCGCGTTGCGTGGCCTGACCCGCGGCGGGCAGC
TCAGCGCGGCACGGGCGCACGACGCCCTGGCCGACTTCGATGCGCTGGCCATCCGGCGGTGGCCCGGTAGCGCGGAACTT
CGGCTGCGGGCGTTCACGCTGCGCGACAATCTGACGGCCTACGACGCGGCTTATGTGGCGCTCGCCGAGGCGTTGGAATG
CGCTGTGCTGACCCGTGACCGGCGACTGGCGCGCTCGGGTGGTCACAGCGTAGAGATCCGTGTCCGCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9258.50 Da
• Isoelectric Point: 10.3629

>AT36140 WP_013828729.1 NZ_AP022562:78863-79117 [Mycobacterium novum]
MSSLLQIRNVPDAVRRELKARAAIQGQSLNAYLLDLISREVNRPTVAEVLQRAAQRAEHASGSAIDVVDADRIERDAQLA
GPRR

Nucleotide

• Length: 255 bp

>AT36140 NZ_AP022562:78863-79117 [Mycobacterium novum]
ATGAGTTCGCTACTTCAGATCCGCAATGTTCCCGACGCGGTTCGTCGGGAACTCAAGGCGCGGGCGGCCATACAGGGCCA
GTCACTGAACGCCTACCTGCTCGACCTGATCAGCCGGGAAGTGAACCGCCCCACAGTCGCCGAAGTCCTGCAGCGCGCCG
CGCAACGCGCCGAGCATGCCAGCGGGTCGGCGATCGATGTAGTCGACGCCGACCGAATCGAGCGGGATGCGCAATTGGCC
GGCCCCCGCAGATGA

Structures

Antitoxin

• AlphaFold DB: A0A7I9Y7W3