Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 3121508..3122144 Replicon chromosome
Accession NZ_AP022557
Organism Geobacillus subterraneus strain E55-1

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag GsuE55_RS15530 Protein ID WP_003253417.1
Coordinates 3121794..3122144 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A679FZL5
Locus tag GsuE55_RS15525 Protein ID WP_033023681.1
Coordinates 3121508..3121789 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GsuE55_RS15505 3117269..3117907 - 639 WP_172418741.1 rhomboid family intramembrane serine protease -
GsuE55_RS15510 3118014..3118403 + 390 WP_033842624.1 holo-ACP synthase -
GsuE55_RS15515 3118483..3119496 + 1014 WP_033842623.1 outer membrane lipoprotein carrier protein LolA -
GsuE55_RS15520 3120227..3121393 + 1167 WP_033842622.1 alanine racemase -
GsuE55_RS15525 3121508..3121789 + 282 WP_033023681.1 hypothetical protein Antitoxin
GsuE55_RS15530 3121794..3122144 + 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GsuE55_RS15535 3122454..3124619 + 2166 WP_033842620.1 RNA-binding transcriptional accessory protein -
GsuE55_RS15540 3124636..3124749 - 114 WP_089135018.1 cortex morphogenetic protein CmpA -
GsuE55_RS15545 3124841..3125305 + 465 WP_033842619.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T36135 WP_003253417.1 NZ_AP022557:3121794-3122144 [Geobacillus subterraneus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T36135 NZ_AP022557:3121794-3122144 [Geobacillus subterraneus]
TTGATTGTCAAACGTGGCGACGTGTATTTTGCGGACCTTTCCCCGGTTGTTGGCTCAGAGCAGGGCGGCGTGCGTCCCGT
CTTGGTCATTCAAAATGACATCGGCAACCGCTTCAGTCCAACGGTAATCGTTGCGGCAATCACGGCGCAAATTCAAAAGG
CAAAATTGCCAACGCATGTGGAGATTGACGCGAAGCGTTACGGGTTTGAACGCGATTCGGTCATTTTGCTTGAGCAAATC
CGTACGATCGATAAGCAACGGCTGACCGATAAAATCACCCATTTGGATGACGAAATGATGGATAAAGTCGATGAAGCGTT
GCAAATCAGTTTAGGGCTGATTGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10669.17 Da        Isoelectric Point: 7.2027

>AT36135 WP_033023681.1 NZ_AP022557:3121508-3121789 [Geobacillus subterraneus]
VSESGATTEIIVRLPQSLVTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT36135 NZ_AP022557:3121508-3121789 [Geobacillus subterraneus]
GTGTCGGAATCGGGCGCAACAACGGAAATCATCGTTCGCTTGCCGCAATCGCTGGTGACGGAGCTGGATGTGCTTGTCAA
ACAGGAAAATGGCAACCGCAATGAACTGATTTATCAGGCGACAAAAATGTATATTCGTGAGCGGAAAAAACGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCAAAAATCAATTTATCTATCGCTTCTGAAGCGTTTCATGCTGAATAC
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A679FZL5

References