Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 2509138..2509832 Replicon chromosome
Accession NZ_AP022557
Organism Geobacillus subterraneus strain E55-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A679G145
Locus tag GsuE55_RS12525 Protein ID WP_033843013.1
Coordinates 2509138..2509524 (-) Length 129 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6N3WIP1
Locus tag GsuE55_RS12530 Protein ID WP_033020681.1
Coordinates 2509524..2509832 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GsuE55_RS12495 2504480..2504677 - 198 WP_172418431.1 hypothetical protein -
GsuE55_RS12500 2504667..2504744 - 78 Protein_2473 helix-turn-helix domain-containing protein -
GsuE55_RS12505 2504818..2505715 - 898 Protein_2474 hypothetical protein -
GsuE55_RS12510 2505712..2506506 - 795 WP_033843017.1 ImmA/IrrE family metallo-endopeptidase -
GsuE55_RS12515 2506742..2507875 + 1134 WP_015375695.1 IS4 family transposase -
GsuE55_RS12520 2508614..2509037 + 424 Protein_2477 IS701 family transposase -
GsuE55_RS12525 2509138..2509524 - 387 WP_033843013.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GsuE55_RS12530 2509524..2509832 - 309 WP_033020681.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GsuE55_RS12535 2510273..2511637 - 1365 Protein_2480 ISLre2 family transposase -
GsuE55_RS12540 2511817..2512308 + 492 Protein_2481 transposase -
GsuE55_RS12545 2512874..2514145 + 1272 WP_033843011.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - wbtL / bpsD / bpsC 2500691..2534829 34138
- inside IScluster/Tn - - 2506742..2511586 4844


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-116)

Antitoxin

SpoVT-AbrB domain

(26-71)


Sequences

Toxin        


Download         Length: 129 a.a.        Molecular weight: 14273.26 Da        Isoelectric Point: 4.6190

>T36134 WP_033843013.1 NZ_AP022557:c2509524-2509138 [Geobacillus subterraneus]
MPADVPERGDFVVLNFNPQAGHEQAGRRNAIVLSPKEFNQATGFIAVCPITNQKKGYPFEVDLPKEGISPDGDGYPITGV
VLTDQIKSLDWKARNLKILKKYNPEDAQIEEIDKIIDECLAKIETYLT

Download         Length: 387 bp

>T36134 NZ_AP022557:c2509524-2509138 [Geobacillus subterraneus]
ATGCCAGCAGACGTACCAGAGAGAGGCGATTTCGTTGTTTTAAACTTCAATCCACAAGCTGGTCATGAGCAGGCTGGGAG
AAGGAATGCTATTGTTTTATCGCCTAAAGAATTCAATCAAGCAACAGGATTTATAGCTGTTTGTCCGATTACCAATCAAA
AGAAGGGTTATCCCTTCGAAGTGGATTTACCAAAAGAAGGAATATCCCCTGATGGCGATGGCTATCCAATAACAGGGGTA
GTTTTAACTGATCAAATTAAATCATTGGATTGGAAAGCTCGTAATCTAAAAATTTTAAAGAAGTATAATCCAGAAGATGC
ACAAATTGAAGAAATAGATAAAATCATTGATGAATGCCTAGCGAAAATAGAAACATATCTGACTTGA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11504.14 Da        Isoelectric Point: 7.2044

>AT36134 WP_033020681.1 NZ_AP022557:c2509832-2509524 [Geobacillus subterraneus]
MNSKEHLESKINQSKGGDYMSTVVAQKWGNSLGIRIPKDVADKIGIKQGSEIELNVIGNEGIITLKPKRTRKKYTLEELI
SQITPENRHEEIDFGIEGRELI

Download         Length: 309 bp

>AT36134 NZ_AP022557:c2509832-2509524 [Geobacillus subterraneus]
ATGAACTCAAAGGAACATTTAGAAAGCAAAATAAACCAAAGTAAAGGAGGGGACTACATGTCTACAGTTGTTGCTCAAAA
ATGGGGAAACAGTTTGGGCATTCGTATCCCAAAAGATGTTGCTGATAAAATAGGGATTAAACAAGGTTCCGAAATAGAAT
TAAATGTGATTGGAAATGAAGGTATCATCACATTAAAGCCAAAAAGAACACGGAAGAAATACACACTGGAAGAACTAATA
TCACAGATTACACCTGAAAATCGACACGAGGAAATTGATTTCGGGATAGAAGGGCGTGAATTGATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A679G145

Antitoxin

Source ID Structure
AlphaFold DB A0A6N3WIP1

References