36133

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 102..624 Replicon plasmid pTHO-015-3
Accession NZ_AP022552
Organism Escherichia coli strain THO-015

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag JLF69_RS25245 Protein ID WP_000220560.1
Coordinates 343..624 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag JLF69_RS25240 Protein ID WP_000121743.1
Coordinates 102..353 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF69_RS25240 102..353 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
JLF69_RS25245 343..624 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF69_RS25250 770..1093 + 324 WP_001181903.1 hypothetical protein -
JLF69_RS25255 1138..1383 + 246 WP_001550454.1 hypothetical protein -
JLF69_RS25260 1373..1588 + 216 WP_001180116.1 hypothetical protein -
JLF69_RS25265 1681..2010 + 330 WP_000866648.1 hypothetical protein -
JLF69_RS25270 2053..2232 + 180 WP_000439078.1 hypothetical protein -
JLF69_RS25275 2302..2451 + 150 WP_000003880.1 hypothetical protein -
JLF69_RS25280 2464..2736 + 273 WP_000160399.1 hypothetical protein -
JLF69_RS25285 2993..3067 + 75 Protein_9 DNA distortion polypeptide 3 -
JLF69_RS25290 3062..3607 + 546 WP_000757693.1 hypothetical protein -
JLF69_RS25295 3610..4776 + 1167 WP_000539529.1 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..46229 46229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T36133 WP_000220560.1 NZ_AP022552:343-624 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T36133 NZ_AP022552:343-624 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT36133 WP_000121743.1 NZ_AP022552:102-353 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT36133 NZ_AP022552:102-353 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References