Detailed information of TA system TA036132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 73043..73644
• Replicon: plasmid pTHO-015-1
• Accession: NZ_AP022550
• Organism: Escherichia coli strain THO-015

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: JLF69_RS24900
• Protein ID: WP_001216034.1
• Coordinates: 73043..73423 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: JLF69_RS24905
• Protein ID: WP_001190712.1
• Coordinates: 73423..73644 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF69_RS24860	68182..69021	-	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
JLF69_RS24865	69015..69362	-	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
JLF69_RS24870	69568..70356	-	789	WP_000503573.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA5	-
JLF69_RS24875	70487..70960	-	474	WP_001389366.1	trimethoprim-resistant dihydrofolate reductase DfrA17	-
JLF69_RS24880	71242..71946	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
JLF69_RS24885	72135..72548	-	414	Protein_90	DDE-type integrase/transposase/recombinase	-
JLF69_RS24890	72472..72831	-	360	WP_001513660.1	hypothetical protein	-
JLF69_RS24895	72859..73038	-	180	WP_001513661.1	hypothetical protein	-
JLF69_RS24900	73043..73423	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JLF69_RS24905	73423..73644	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JLF69_RS24910	73827..75383	+	1557	WP_001617892.1	type I restriction-modification system subunit M	-
JLF69_RS24915	75380..76651	+	1272	WP_023142242.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5	-	1..88121	88121
	-	inside	IScluster/Tn	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5	-	44070..72509	28439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T36132 WP_001216034.1 NZ_AP022550:c73423-73043 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T36132 NZ_AP022550:c73423-73043 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT36132 WP_001190712.1 NZ_AP022550:c73644-73423 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT36132 NZ_AP022550:c73644-73423 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6