Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4274998..4275833 Replicon chromosome
Accession NZ_AP022549
Organism Escherichia coli strain THO-015

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag JLF69_RS20785 Protein ID WP_000854759.1
Coordinates 4274998..4275375 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag JLF69_RS20790 Protein ID WP_001295723.1
Coordinates 4275465..4275833 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF69_RS20760 4271109..4272749 - 1641 WP_001332039.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -
JLF69_RS20765 4273522..4273698 - 177 Protein_4073 helix-turn-helix domain-containing protein -
JLF69_RS20770 4274062..4274220 - 159 WP_001467148.1 hypothetical protein -
JLF69_RS20775 4274320..4274496 - 177 WP_000839286.1 DUF957 domain-containing protein -
JLF69_RS20780 4274513..4275001 - 489 WP_000761690.1 hypothetical protein -
JLF69_RS20785 4274998..4275375 - 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
JLF69_RS20790 4275465..4275833 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
JLF69_RS20795 4275996..4276217 - 222 WP_000692345.1 DUF987 domain-containing protein -
JLF69_RS20800 4276280..4276756 - 477 WP_001186775.1 RadC family protein -
JLF69_RS20805 4276772..4277245 - 474 WP_001350782.1 antirestriction protein -
JLF69_RS20810 4277587..4278405 - 819 WP_001234738.1 DUF945 domain-containing protein -
JLF69_RS20815 4278560..4278718 - 159 WP_001323397.1 DUF905 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4263352..4292613 29261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T36120 WP_000854759.1 NZ_AP022549:c4275375-4274998 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T36120 NZ_AP022549:c4275375-4274998 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT36120 WP_001295723.1 NZ_AP022549:c4275833-4275465 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT36120 NZ_AP022549:c4275833-4275465 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6


Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References