Detailed information of TA system TA036093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 49420..49979
• Replicon: plasmid pTHO-011-1
• Accession: NZ_AP022548
• Organism: Klebsiella michiganensis strain THO-011

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: JLG71_RS27970
• Protein ID: WP_000421259.1
• Coordinates: 49704..49979 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: JLG71_RS27965
• Protein ID: WP_001178087.1
• Coordinates: 49420..49704 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLG71_RS27945	45069..45602	-	534	WP_001372168.1	transcription termination factor NusG	-
JLG71_RS27950	46044..46331	-	288	WP_000074849.1	conjugal transfer protein TraA	-
JLG71_RS27955	46249..46512	-	264	WP_001324596.1	ash family protein	-
JLG71_RS27960	47485..48516	+	1032	WP_000907852.1	replication initiation protein	-
JLG71_RS27965	49420..49704	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
JLG71_RS27970	49704..49979	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLG71_RS27975	50085..50366	+	282	WP_001105066.1	hypothetical protein	-
JLG71_RS27980	50558..50824	-	267	WP_000627832.1	hypothetical protein	-
JLG71_RS27985	50824..51318	-	495	WP_015058884.1	hypothetical protein	-
JLG71_RS27990	51374..51976	-	603	WP_061671620.1	proQ/FINO family protein	-
JLG71_RS27995	52330..53676	+	1347	WP_001132027.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-8	-	1..106439	106439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T36093 WP_000421259.1 NZ_AP022548:49704-49979 [Klebsiella michiganensis]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T36093 NZ_AP022548:49704-49979 [Klebsiella michiganensis]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT36093 WP_001178087.1 NZ_AP022548:49420-49704 [Klebsiella michiganensis]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT36093 NZ_AP022548:49420-49704 [Klebsiella michiganensis]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625