Detailed information of TA system TA036092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5209667..5210243
• Replicon: chromosome
• Accession: NZ_AP022547
• Organism: Klebsiella michiganensis strain THO-011

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: JLG71_RS24400
• Protein ID: WP_200914330.1
• Coordinates: 5209956..5210243 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: JLG71_RS24395
• Protein ID: WP_200913862.1
• Coordinates: 5209667..5209969 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLG71_RS24380	5205725..5206705	-	981	WP_032747738.1	IS110 family transposase	-
JLG71_RS24385	5207295..5208206	+	912	WP_116281053.1	acetamidase/formamidase family protein	-
JLG71_RS24390	5208203..5209546	+	1344	WP_004128506.1	APC family permease	-
JLG71_RS24395	5209667..5209969	-	303	WP_200913862.1	BrnA antitoxin family protein	Antitoxin
JLG71_RS24400	5209956..5210243	-	288	WP_200914330.1	BrnT family toxin	Toxin
JLG71_RS24405	5210503..5210946	-	444	WP_200913863.1	FosA family fosfomycin resistance glutathione transferase	-
JLG71_RS24410	5210940..5211848	-	909	WP_032747747.1	LysR family transcriptional regulator	-
JLG71_RS24415	5211936..5212718	+	783	WP_116281052.1	NAD(P)H-dependent oxidoreductase	-
JLG71_RS24420	5212866..5213450	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
JLG71_RS24425	5213596..5214396	+	801	WP_046878031.1	winged helix-turn-helix domain-containing protein	-
JLG71_RS24430	5214393..5214911	+	519	WP_004098240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IS/Tn	fosA	-	5205725..5210946	5221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (17-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11279.80 Da
• Isoelectric Point: 8.5899

>T36092 WP_200914330.1 NZ_AP022547:c5210243-5209956 [Klebsiella michiganensis]
MPMEFEWDANKAIRNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T36092 NZ_AP022547:c5210243-5209956 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAATAAGGAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11572.18 Da
• Isoelectric Point: 9.7963

>AT36092 WP_200913862.1 NZ_AP022547:c5209969-5209667 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRADELKALANKTDDEIDYSDIPATDDAHWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT36092 NZ_AP022547:c5209969-5209667 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGCCGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACATTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG