Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4546505..4547124 Replicon chromosome
Accession NZ_AP022547
Organism Klebsiella michiganensis strain THO-011

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag JLG71_RS21355 Protein ID WP_004099646.1
Coordinates 4546906..4547124 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0J2I7Z1
Locus tag JLG71_RS21350 Protein ID WP_025107145.1
Coordinates 4546505..4546879 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLG71_RS21340 4541661..4542854 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JLG71_RS21345 4542877..4546023 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
JLG71_RS21350 4546505..4546879 + 375 WP_025107145.1 Hha toxicity modulator TomB Antitoxin
JLG71_RS21355 4546906..4547124 + 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
JLG71_RS21360 4547287..4547853 + 567 WP_064357999.1 maltose O-acetyltransferase -
JLG71_RS21365 4547980..4548450 + 471 WP_014228205.1 YlaC family protein -
JLG71_RS21370 4548425..4549879 - 1455 WP_116280241.1 PLP-dependent aminotransferase family protein -
JLG71_RS21375 4549981..4550679 + 699 WP_116280242.1 GNAT family N-acetyltransferase -
JLG71_RS21380 4550676..4550816 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
JLG71_RS21385 4550816..4551079 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T36090 WP_004099646.1 NZ_AP022547:4546906-4547124 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T36090 NZ_AP022547:4546906-4547124 [Klebsiella michiganensis]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGTTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCTGAGC
TGACGATGAATAAACTGTATGACAAGATCCCCCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14366.15 Da        Isoelectric Point: 4.8989

>AT36090 WP_025107145.1 NZ_AP022547:4546505-4546879 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNACRENPASLSC

Download         Length: 375 bp

>AT36090 NZ_AP022547:4546505-4546879 [Klebsiella michiganensis]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATTACCCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGCGCAGATCTGCAGAAATGGCGTAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCTGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2I7Z1

References