Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2026817..2027407 Replicon chromosome
Accession NZ_AP022547
Organism Klebsiella michiganensis strain THO-011

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag JLG71_RS09570 Protein ID WP_014229979.1
Coordinates 2027075..2027407 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag JLG71_RS09565 Protein ID WP_004852307.1
Coordinates 2026817..2027074 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLG71_RS09540 2022074..2022682 - 609 WP_057174013.1 glutathione S-transferase N-terminal domain-containing protein -
JLG71_RS09545 2022859..2023785 + 927 WP_014838892.1 LysR family transcriptional regulator -
JLG71_RS09550 2023823..2024821 - 999 WP_200914153.1 aldo/keto reductase -
JLG71_RS09555 2024922..2025836 + 915 WP_025106070.1 LysR family transcriptional regulator -
JLG71_RS09560 2026350..2026619 + 270 WP_116281084.1 helix-turn-helix domain-containing protein -
JLG71_RS09565 2026817..2027074 + 258 WP_004852307.1 antitoxin Antitoxin
JLG71_RS09570 2027075..2027407 + 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
JLG71_RS09575 2027404..2027610 - 207 WP_200914161.1 hypothetical protein -
JLG71_RS09580 2027777..2028976 - 1200 WP_039493160.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T36081 WP_014229979.1 NZ_AP022547:2027075-2027407 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T36081 NZ_AP022547:2027075-2027407 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCAGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT36081 WP_004852307.1 NZ_AP022547:2026817-2027074 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT36081 NZ_AP022547:2026817-2027074 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References