Detailed information of TA system TA036080

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1338669..1339452
• Replicon: chromosome
• Accession: NZ_AP022547
• Organism: Klebsiella michiganensis strain THO-011

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JLG71_RS06455
• Protein ID: WP_010434197.1
• Coordinates: 1338669..1339043 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JLG71_RS06460
• Protein ID: WP_024274583.1
• Coordinates: 1339093..1339452 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLG71_RS06435	1334341..1334574	-	234	WP_004132711.1	hypothetical protein	-
JLG71_RS06440	1334984..1335256	+	273	WP_016247220.1	hypothetical protein	-
JLG71_RS06445	1335459..1336367	+	909	WP_024274585.1	kdo(2)-lipid IV(A) palmitoleoyltransferase	-
JLG71_RS06450	1337141..1338130	+	990	WP_010434201.1	glycosyltransferase	-
JLG71_RS06455	1338669..1339043	-	375	WP_010434197.1	TA system toxin CbtA family protein	Toxin
JLG71_RS06460	1339093..1339452	-	360	WP_024274583.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLG71_RS06465	1339475..1339696	-	222	WP_010434193.1	DUF987 domain-containing protein	-
JLG71_RS06470	1339710..1340192	-	483	WP_024274581.1	DNA repair protein RadC	-
JLG71_RS06475	1340189..1340422	-	234	WP_010434188.1	hypothetical protein	-
JLG71_RS06480	1340433..1340912	-	480	WP_010434186.1	antirestriction protein	-
JLG71_RS06485	1340991..1341812	-	822	WP_185219886.1	DUF945 domain-containing protein	-
JLG71_RS06490	1341923..1342135	-	213	WP_010434180.1	hypothetical protein	-
JLG71_RS06495	1342216..1342575	-	360	WP_010434179.1	hypothetical protein	-
JLG71_RS06500	1342630..1343100	-	471	WP_200914022.1	hypothetical protein	-
JLG71_RS06505	1343378..1344235	-	858	WP_010434177.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1335087..1365671	30584

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (8-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13977.96 Da
• Isoelectric Point: 9.6955

>T36080 WP_010434197.1 NZ_AP022547:c1339043-1338669 [Klebsiella michiganensis]
MQIHSLFLQRAASSRLSSVEIWQKLLEYLLEQHYGLTLNDTPFGNDSVIQKHIDAGISLYDAVNFIVEKYDLVRTDRHGF
SVKEQSPFIGSLDILRARKATGLMTRKGYKTVTDITGGRFSGGK

Nucleotide

• Length: 375 bp

>T36080 NZ_AP022547:c1339043-1338669 [Klebsiella michiganensis]
ATGCAAATACATTCCCTGTTCCTTCAAAGGGCGGCGTCGTCACGTCTGTCGTCAGTCGAAATCTGGCAAAAATTACTGGA
GTATCTGCTGGAGCAACACTACGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAACACATTG
ATGCGGGAATCTCACTGTATGACGCTGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTC
AGCGTGAAAGAGCAGTCTCCTTTTATCGGCAGCCTTGATATTCTCCGAGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13469.67 Da
• Isoelectric Point: 7.7511

>AT36080 WP_024274583.1 NZ_AP022547:c1339452-1339093 [Klebsiella michiganensis]
MKKATRVINHNITEPWWGLRRNITPCFGARLVQEGNHLHYLADRASIAGTFNDADLRHLDQVFPVLMKQMELMLASSELT
PRIQRCITLHVKGLICEADTLGSCGYLYIVIYPASVTTE

Nucleotide

• Length: 360 bp

>AT36080 NZ_AP022547:c1339452-1339093 [Klebsiella michiganensis]
ATGAAGAAAGCAACCCGTGTAATCAACCATAACATCACGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
TGGTGCACGGCTGGTACAGGAGGGTAACCACCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACG
CAGATTTACGCCATCTGGACCAGGTATTTCCGGTACTGATGAAGCAGATGGAGCTTATGCTGGCCAGCAGCGAACTCACG
CCCCGCATTCAGCGCTGCATCACGCTCCACGTAAAAGGGCTGATATGTGAGGCCGATACACTCGGCTCCTGCGGTTATTT
GTACATCGTAATTTATCCGGCGTCTGTAACAACCGAATAA