Detailed information of TA system TA036079

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 917314..918140
• Replicon: chromosome
• Accession: NZ_AP022547
• Organism: Klebsiella michiganensis strain THO-011

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: JLG71_RS04455
• Protein ID: WP_128878862.1
• Coordinates: 917751..918140 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: JLG71_RS04450
• Protein ID: WP_131930492.1
• Coordinates: 917314..917694 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLG71_RS04420	913855..914487	-	633	WP_131930487.1	inovirus Gp2 family protein	-
JLG71_RS04425	915028..915852	+	825	WP_131930488.1	DUF945 domain-containing protein	-
JLG71_RS04430	915858..916106	+	249	WP_131930489.1	DUF905 domain-containing protein	-
JLG71_RS04435	916141..916584	+	444	WP_131930490.1	antirestriction protein	-
JLG71_RS04440	916596..917075	+	480	WP_131930491.1	DNA repair protein RadC	-
JLG71_RS04445	917089..917310	+	222	WP_109241248.1	DUF987 domain-containing protein	-
JLG71_RS04450	917314..917694	+	381	WP_131930492.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLG71_RS04455	917751..918140	+	390	WP_128878862.1	TA system toxin CbtA family protein	Toxin
JLG71_RS04460	918227..919066	+	840	WP_200913992.1	DUF4942 domain-containing protein	-
JLG71_RS04465	919739..920737	+	999	WP_025106003.1	aldo/keto reductase	-
JLG71_RS04470	920826..922001	-	1176	WP_200913993.1	acetyl-CoA C-acetyltransferase	-
JLG71_RS04475	921998..922411	-	414	WP_200913994.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	875661..926752	51091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (22-121)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14420.54 Da
• Isoelectric Point: 9.8629

>T36079 WP_128878862.1 NZ_AP022547:917751-918140 [Klebsiella michiganensis]
MQTKSSPLMRAASSRPSPVYIWQTLLTSLLAHHYGLTLSDTPFSDEQVIQQHIDAGISLVDALNFIVEKYELVRTDRPGF
SILTQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T36079 NZ_AP022547:917751-918140 [Klebsiella michiganensis]
ATGCAAACCAAATCATCCCCCCTCATGCGGGCGGCATCGTCACGTCCGTCTCCCGTGTATATCTGGCAGACCCTGCTGAC
CAGCCTGCTGGCACACCACTATGGCCTCACGCTTAGCGATACGCCGTTCAGTGATGAACAGGTCATTCAGCAACATATTG
ATGCCGGTATCTCGCTGGTTGATGCCCTGAATTTTATCGTTGAGAAGTATGAACTGGTCCGCACTGACCGCCCAGGCTTC
AGCATCCTGACGCAGTCTCCGTTTATCACACCCATAGATATCCTCCGGGCCAGGAAAGCAACGGGGCTGATGAACCGTGA
CACGTATAAAGAGGTGACGGCTATTACCAGAGGGCAACATCCGCAGGTCAGCGCTCCAGGCAAGCGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 14178.29 Da
• Isoelectric Point: 7.3713

>AT36079 WP_131930492.1 NZ_AP022547:917314-917694 [Klebsiella michiganensis]
MIKESYILSKKTPVTTHDISQPWWGLRRSVSPCFGARLVLEGNRLHYLADRANFDGQFCDADLRHLDQAFPVVMKQLELM
LTSGELNPRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVATA

Nucleotide

• Length: 381 bp

>AT36079 NZ_AP022547:917314-917694 [Klebsiella michiganensis]
ATGATTAAGGAGAGCTACATTTTGTCGAAGAAAACCCCGGTAACAACGCATGATATCAGCCAACCCTGGTGGGGGCTCAG
ACGCAGTGTTTCCCCGTGTTTTGGAGCCCGTCTGGTCCTGGAGGGAAACCGCCTGCATTACCTGGCTGACCGGGCAAACT
TTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCCTTCCCGGTAGTGATGAAACAGCTGGAGTTAATG
CTCACCAGCGGTGAACTGAATCCCCGTCATCAGCACTGCGTCACGTTGTATGCAAAAGGTCTGACCTGCGAGGCCGATAC
GCTCGGTTCGCATGGCTATGTTTATATCGCGATTTATCCCACTCCCGTCGCCACGGCATAA