Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 666258..666850 Replicon chromosome
Accession NZ_AP022547
Organism Klebsiella michiganensis strain THO-011

Toxin (Protein)


Gene name doc Uniprot ID A0A7H4PHG5
Locus tag JLG71_RS03245 Protein ID WP_004105955.1
Coordinates 666476..666850 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A2J4YM08
Locus tag JLG71_RS03240 Protein ID WP_004105957.1
Coordinates 666258..666479 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLG71_RS03220 661815..662369 + 555 WP_014226934.1 YgjV family protein -
JLG71_RS03225 662388..663635 - 1248 WP_004105963.1 serine/threonine transporter SstT -
JLG71_RS03230 663890..664858 - 969 WP_048260487.1 TerC family protein -
JLG71_RS03235 665109..666098 - 990 WP_057173194.1 Gfo/Idh/MocA family oxidoreductase -
JLG71_RS03240 666258..666479 + 222 WP_004105957.1 type II toxin-antitoxin system prevent-host-death family antitoxin Antitoxin
JLG71_RS03245 666476..666850 + 375 WP_004105955.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
JLG71_RS03250 666830..667333 - 504 WP_025108311.1 M48 family metallopeptidase -
JLG71_RS03255 667412..669055 - 1644 WP_200913966.1 glycoside hydrolase family 43 protein -
JLG71_RS03260 669184..670050 - 867 WP_102803972.1 AraC family transcriptional regulator -
JLG71_RS03265 670158..671501 + 1344 WP_200913967.1 glycoside-pentoside-hexuronide (GPH):cation symporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(5-119)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-52)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13895.93 Da        Isoelectric Point: 6.0769

>T36077 WP_004105955.1 NZ_AP022547:666476-666850 [Klebsiella michiganensis]
MKWVSASEVIAFHDRILQHLPGVKGMSDPGRAEAIIYRVQNRFHYEGVNDIFELAATYWVAIARGHIFNDGNKRTAFFIT
MTFLARNGYLIADEDTRLEELTVLAATGEATVVVLADALRQLAL

Download         Length: 375 bp

>T36077 NZ_AP022547:666476-666850 [Klebsiella michiganensis]
ATGAAATGGGTGAGCGCAAGCGAAGTCATTGCCTTTCATGATCGCATTTTACAACATCTCCCCGGCGTTAAAGGTATGTC
CGATCCTGGTCGGGCGGAAGCCATAATTTATCGTGTACAGAACCGCTTTCACTATGAAGGGGTGAACGACATTTTTGAAC
TGGCTGCAACCTATTGGGTCGCTATCGCGCGTGGGCACATTTTTAATGATGGTAATAAGAGGACTGCTTTTTTTATCACC
ATGACGTTCCTGGCCCGCAATGGGTATCTTATCGCTGATGAAGATACCCGGTTGGAAGAACTCACTGTGCTGGCTGCGAC
AGGCGAAGCTACGGTAGTGGTTCTTGCTGATGCGTTACGGCAATTGGCGCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8185.19 Da        Isoelectric Point: 5.7996

>AT36077 WP_004105957.1 NZ_AP022547:666258-666479 [Klebsiella michiganensis]
MRSYSTSEARQNIAAVMDAAASGEPIEITRRDGTSNVLISKAEYETFMNAKLDAEFDFIMQRHGRTVKALTDR

Download         Length: 222 bp

>AT36077 NZ_AP022547:666258-666479 [Klebsiella michiganensis]
ATGCGCTCATACAGTACATCTGAAGCCAGACAGAATATTGCGGCCGTCATGGACGCCGCTGCCAGCGGAGAACCGATTGA
AATTACACGGCGTGATGGAACGTCTAATGTTTTGATCAGTAAGGCGGAATATGAAACGTTTATGAATGCCAAACTTGATG
CGGAGTTTGATTTTATTATGCAGCGACACGGCCGCACAGTTAAGGCGTTGACCGATCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H4PHG5

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4YM08

References