Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4353637..4354232 Replicon chromosome
Accession NZ_AP022540
Organism Escherichia coli strain THO-010

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag JLH08_RS20415 Protein ID WP_000239579.1
Coordinates 4353637..4353987 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag JLH08_RS20420 Protein ID WP_001223208.1
Coordinates 4353981..4354232 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLH08_RS20395 4349083..4350105 - 1023 WP_001313531.1 ABC transporter permease -
JLH08_RS20400 4350119..4351621 - 1503 WP_021557471.1 sugar ABC transporter ATP-binding protein -
JLH08_RS20405 4351761..4352717 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
JLH08_RS20410 4353027..4353557 + 531 WP_000055072.1 inorganic diphosphatase -
JLH08_RS20415 4353637..4353987 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
JLH08_RS20420 4353981..4354232 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
JLH08_RS20425 4354445..4354786 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
JLH08_RS20430 4354789..4358568 - 3780 WP_021557470.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T36060 WP_000239579.1 NZ_AP022540:c4353987-4353637 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T36060 NZ_AP022540:c4353987-4353637 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT36060 WP_001223208.1 NZ_AP022540:c4354232-4353981 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT36060 NZ_AP022540:c4354232-4353981 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References