Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1086563..1087146 Replicon chromosome
Accession NZ_AP022540
Organism Escherichia coli strain THO-010

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag JLH08_RS05115 Protein ID WP_000254745.1
Coordinates 1086811..1087146 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1M2U551
Locus tag JLH08_RS05110 Protein ID WP_021557150.1
Coordinates 1086563..1086811 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLH08_RS05100 1082902..1084203 + 1302 WP_021557151.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JLH08_RS05105 1084251..1086485 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JLH08_RS05110 1086563..1086811 + 249 WP_021557150.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JLH08_RS05115 1086811..1087146 + 336 WP_000254745.1 endoribonuclease MazF Toxin
JLH08_RS05120 1087217..1088008 + 792 WP_001071643.1 nucleoside triphosphate pyrophosphohydrolase -
JLH08_RS05125 1088236..1089873 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JLH08_RS05130 1089961..1091259 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T36044 WP_000254745.1 NZ_AP022540:1086811-1087146 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T36044 NZ_AP022540:1086811-1087146 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9383.79 Da        Isoelectric Point: 4.4665

>AT36044 WP_021557150.1 NZ_AP022540:1086563-1086811 [Escherichia coli]
MIHSSVKRWGNSPAVRIPVTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT36044 NZ_AP022540:1086563-1086811 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGTTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2U551

References