Detailed information of TA system TA036039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 132992..133586
• Replicon: plasmid pTHO-008-1
• Accession: NZ_AP022537
• Organism: Escherichia coli strain THO-008

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JLF34_RS24865
• Protein ID: WP_001403920.1
• Coordinates: 132992..133267 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: JLF34_RS24870
• Protein ID: WP_001261274.1
• Coordinates: 133356..133586 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF34_RS24830	128073..128380	+	308	Protein_158	type II toxin-antitoxin system ParD family antitoxin	-
JLF34_RS24835	128723..128905	+	183	WP_042004909.1	hypothetical protein	-
JLF34_RS24840	129026..129766	+	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
JLF34_RS24845	130089..131078	-	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
JLF34_RS24850	131966..132235	+	270	WP_001275013.1	DUF1778 domain-containing protein	-
JLF34_RS24855	132239..132769	+	531	WP_000348883.1	GNAT family N-acetyltransferase	-
JLF34_RS24860	132770..132987	+	218	Protein_164	transposase	-
JLF34_RS24865	132992..133267	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF34_RS24870	133356..133586	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JLF34_RS24875	133785..135959	-	2175	Protein_167	histidinol-phosphatase	-
JLF34_RS24880	136024..136779	-	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
JLF34_RS24885	136776..138275	-	1500	WP_000174402.1	IS21-like element ISEc10 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..151109	151109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T36039 WP_001403920.1 NZ_AP022537:c133267-132992 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T36039 NZ_AP022537:c133267-132992 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT36039 WP_001261274.1 NZ_AP022537:c133586-133356 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT36039 NZ_AP022537:c133586-133356 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1