Detailed information of TA system TA036038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 131966..132769
• Replicon: plasmid pTHO-008-1
• Accession: NZ_AP022537
• Organism: Escherichia coli strain THO-008

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: JLF34_RS24855
• Protein ID: WP_000348883.1
• Coordinates: 132239..132769 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: JLF34_RS24850
• Protein ID: WP_001275013.1
• Coordinates: 131966..132235 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF34_RS24830	128073..128380	+	308	Protein_158	type II toxin-antitoxin system ParD family antitoxin	-
JLF34_RS24835	128723..128905	+	183	WP_042004909.1	hypothetical protein	-
JLF34_RS24840	129026..129766	+	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
JLF34_RS24845	130089..131078	-	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
JLF34_RS24850	131966..132235	+	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
JLF34_RS24855	132239..132769	+	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
JLF34_RS24860	132770..132987	+	218	Protein_164	transposase	-
JLF34_RS24865	132992..133267	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
JLF34_RS24870	133356..133586	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JLF34_RS24875	133785..135959	-	2175	Protein_167	histidinol-phosphatase	-
JLF34_RS24880	136024..136779	-	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..151109	151109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T36038 WP_000348883.1 NZ_AP022537:132239-132769 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T36038 NZ_AP022537:132239-132769 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT36038 WP_001275013.1 NZ_AP022537:131966-132235 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT36038 NZ_AP022537:131966-132235 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7