Detailed information of TA system TA036033

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4393649..4394481
• Replicon: chromosome
• Accession: NZ_AP022536
• Organism: Escherichia coli strain THO-008

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: JLF34_RS20870
• Protein ID: WP_000854765.1
• Coordinates: 4393649..4394023 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: JLF34_RS20875
• Protein ID: WP_001295723.1
• Coordinates: 4394113..4394481 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF34_RS20845	4389055..4389234	+	180	WP_001315171.1	hypothetical protein	-
JLF34_RS20850	4389633..4391669	-	2037	Protein_4073	hypothetical protein	-
JLF34_RS20855	4392710..4392868	-	159	WP_001467148.1	hypothetical protein	-
JLF34_RS20860	4392968..4393144	-	177	WP_001586605.1	DUF957 domain-containing protein	-
JLF34_RS20865	4393161..4393652	-	492	WP_001586604.1	hypothetical protein	-
JLF34_RS20870	4393649..4394023	-	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
JLF34_RS20875	4394113..4394481	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF34_RS20880	4394644..4394865	-	222	WP_000692300.1	DUF987 domain-containing protein	-
JLF34_RS20885	4394928..4395404	-	477	WP_001186773.1	RadC family protein	-
JLF34_RS20890	4395420..4395893	-	474	WP_001387789.1	antirestriction protein	-
JLF34_RS20895	4396059..4396232	-	174	WP_001183321.1	hypothetical protein	-
JLF34_RS20900	4396232..4397050	-	819	WP_001234716.1	DUF945 domain-containing protein	-
JLF34_RS20905	4397205..4397363	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T36033 WP_000854765.1 NZ_AP022536:c4394023-4393649 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T36033 NZ_AP022536:c4394023-4393649 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT36033 WP_001295723.1 NZ_AP022536:c4394481-4394113 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT36033 NZ_AP022536:c4394481-4394113 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52