Detailed information of TA system TA036031

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4208648..4209072
• Replicon: chromosome
• Accession: NZ_AP022536
• Organism: Escherichia coli strain THO-008

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: JLF34_RS20040
• Protein ID: WP_001586653.1
• Coordinates: 4208648..4208836 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: JLF34_RS20045
• Protein ID: WP_000125566.1
• Coordinates: 4208839..4209072 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF34_RS20010	4204210..4205496	+	1287	WP_000800470.1	peptidylprolyl isomerase SurA	-
JLF34_RS20015	4205496..4206485	+	990	WP_000241280.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
JLF34_RS20020	4206482..4207303	+	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
JLF34_RS20025	4207306..4207683	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
JLF34_RS20030	4207690..4208538	+	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
JLF34_RS20035	4208535..4208651	-	117	WP_001389634.1	CcdB family protein	-
JLF34_RS20040	4208648..4208836	-	189	WP_001586653.1	CcdB family protein	Toxin
JLF34_RS20045	4208839..4209072	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JLF34_RS20050	4209158..4209637	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
JLF34_RS20055	4209829..4211691	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
JLF34_RS20060	4211684..4212214	-	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
JLF34_RS20065	4212322..4213653	-	1332	WP_001183198.1	MFS transporter	-
JLF34_RS20070	4213712..4213999	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7029.22 Da
• Isoelectric Point: 8.7938

>T36031 WP_001586653.1 NZ_AP022536:c4208836-4208648 [Escherichia coli]
MQFTVYRSRGSNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T36031 NZ_AP022536:c4208836-4208648 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGTAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCAGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCTCCAGAACGGCTTAACA
CAATCCTTTTACTGGTAGACGGCTCATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT36031 WP_000125566.1 NZ_AP022536:c4209072-4208839 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT36031 NZ_AP022536:c4209072-4208839 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9