Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2610693..2611331 Replicon chromosome
Accession NZ_AP022536
Organism Escherichia coli strain THO-008

Toxin (Protein)


Gene name hicA Uniprot ID D3GRG9
Locus tag JLF34_RS12265 Protein ID WP_001314712.1
Coordinates 2611155..2611331 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JLF34_RS12260 Protein ID WP_077248684.1
Coordinates 2610693..2611109 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF34_RS12240 2605845..2606786 - 942 WP_001587036.1 ABC transporter permease -
JLF34_RS12245 2606787..2607800 - 1014 WP_000220407.1 ABC transporter ATP-binding protein -
JLF34_RS12250 2607818..2608963 - 1146 WP_000047443.1 ABC transporter substrate-binding protein -
JLF34_RS12255 2609208..2610614 - 1407 WP_000760623.1 PLP-dependent aminotransferase family protein -
JLF34_RS12260 2610693..2611109 - 417 WP_077248684.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JLF34_RS12265 2611155..2611331 - 177 WP_001314712.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JLF34_RS12270 2611553..2611783 + 231 WP_000494241.1 YncJ family protein -
JLF34_RS12275 2611875..2613836 - 1962 WP_001587031.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JLF34_RS12280 2613909..2614445 - 537 WP_000429149.1 DNA-binding transcriptional regulator SutR -
JLF34_RS12285 2614498..2615709 + 1212 WP_071593600.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(87-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6746.81 Da        Isoelectric Point: 11.8888

>T36025 WP_001314712.1 NZ_AP022536:c2611331-2611155 [Escherichia coli]
VKQSEFRRWLESQGVNVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T36025 NZ_AP022536:c2611331-2611155 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCAATGTAGCAAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTACGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15232.60 Da        Isoelectric Point: 4.7286

>AT36025 WP_077248684.1 NZ_AP022536:c2611109-2610693 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSDITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT36025 NZ_AP022536:c2611109-2610693 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGACATCACTCAGCAAGAATTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GRG9

Antitoxin

Source ID Structure

References