Detailed information of TA system TA035986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4306614..4307434
• Replicon: chromosome
• Accession: NZ_AP022533
• Organism: Escherichia coli strain THO-006

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: JLF32_RS20700
• Protein ID: WP_001054379.1
• Coordinates: 4306614..4306871 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: JLF32_RS20705
• Protein ID: WP_000123957.1
• Coordinates: 4306883..4307434 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF32_RS20680	4302161..4303141	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
JLF32_RS20685	4303724..4304830	-	1107	Protein_4051	DNA helicase	-
JLF32_RS20690	4304893..4306040	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
JLF32_RS20695	4306073..4306237	+	165	Protein_4053	GNAT family N-acetyltransferase	-
JLF32_RS20700	4306614..4306871	+	258	WP_001054379.1	YjhX family toxin	Toxin
JLF32_RS20705	4306883..4307434	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
JLF32_RS20710	4307486..4308232	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
JLF32_RS20715	4308360..4308620	+	261	WP_000077645.1	hypothetical protein	-
JLF32_RS20720	4308658..4308774	+	117	Protein_4058	VOC family protein	-
JLF32_RS20725	4309019..4310140	+	1122	WP_000010833.1	M42 family metallopeptidase	-
JLF32_RS20730	4310137..4310415	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
JLF32_RS20735	4310427..4311740	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T35986 WP_001054379.1 NZ_AP022533:4306614-4306871 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T35986 NZ_AP022533:4306614-4306871 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT35986 WP_000123957.1 NZ_AP022533:4306883-4307434 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT35986 NZ_AP022533:4306883-4307434 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030