Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3659438..3660056 Replicon chromosome
Accession NZ_AP022533
Organism Escherichia coli strain THO-006

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JLF32_RS17665 Protein ID WP_001291435.1
Coordinates 3659838..3660056 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JLF32_RS17660 Protein ID WP_000344800.1
Coordinates 3659438..3659812 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF32_RS17650 3654528..3655721 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JLF32_RS17655 3655744..3658893 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
JLF32_RS17660 3659438..3659812 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JLF32_RS17665 3659838..3660056 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JLF32_RS17670 3660229..3660780 + 552 WP_000102539.1 maltose O-acetyltransferase -
JLF32_RS17675 3660896..3661366 + 471 WP_001304825.1 YlaC family protein -
JLF32_RS17680 3661530..3663080 + 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JLF32_RS17685 3663122..3663475 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JLF32_RS17695 3663854..3664165 + 312 WP_000409908.1 MGMT family protein -
JLF32_RS17700 3664196..3664768 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35980 WP_001291435.1 NZ_AP022533:3659838-3660056 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T35980 NZ_AP022533:3659838-3660056 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT35980 WP_000344800.1 NZ_AP022533:3659438-3659812 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT35980 NZ_AP022533:3659438-3659812 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References