Detailed information of TA system TA035978

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2597710..2598710
• Replicon: chromosome
• Accession: NZ_AP022533
• Organism: Escherichia coli strain THO-006

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: JLF32_RS12380
• Protein ID: WP_200923998.1
• Coordinates: 2597976..2598710 (+)
• Length: 245 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: V0VAH2
• Locus tag: JLF32_RS12375
• Protein ID: WP_001304368.1
• Coordinates: 2597710..2597976 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF32_RS12360	2594078..2595529	+	1452	WP_000854660.1	tagaturonate reductase	-
JLF32_RS12365	2595726..2596640	+	915	WP_001443133.1	bestrophin family protein	-
JLF32_RS12370	2596644..2597402	-	759	WP_001286540.1	trans-aconitate 2-methyltransferase	-
JLF32_RS12375	2597710..2597976	+	267	WP_001304368.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
JLF32_RS12380	2597976..2598710	+	735	WP_200923998.1	HipA N-terminal domain-containing protein	Toxin
JLF32_RS12385	2598790..2599770	+	981	WP_200923999.1	type II toxin-antitoxin system HipA family toxin	-
JLF32_RS12390	2600029..2600442	-	414	WP_000753076.1	acetylxylan esterase	-
JLF32_RS12395	2600490..2600789	-	300	Protein_2429	LysR family transcriptional regulator	-
JLF32_RS12400	2600780..2601103	+	324	Protein_2430	SDR family NAD(P)-dependent oxidoreductase	-
JLF32_RS12405	2601879..2602442	+	564	WP_001327671.1	type 1 fimbrial major subunit FimA	-
JLF32_RS12410	2602804..2603514	+	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-239)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 245 a.a.
• Molecular weight: 27529.81 Da
• Isoelectric Point: 8.2518

>T35978 WP_200923998.1 NZ_AP022533:2597976-2598710 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRF
QTTV

Nucleotide

• Length: 735 bp

>T35978 NZ_AP022533:2597976-2598710 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGATAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTTC
CAGACAACCGTTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9957.31 Da
• Isoelectric Point: 4.9632

>AT35978 WP_001304368.1 NZ_AP022533:2597710-2597976 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Nucleotide

• Length: 267 bp

>AT35978 NZ_AP022533:2597710-2597976 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
GCAGAACAGCAAGATTTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: V0VAH2