Detailed information of TA system TA035962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 35078..35600
• Replicon: plasmid pTHO-004-1
• Accession: NZ_AP022528
• Organism: Klebsiella pneumoniae strain THO-004

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: JLF70_RS25625
• Protein ID: WP_004187019.1
• Coordinates: 35316..35600 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: JLF70_RS25620
• Protein ID: WP_004187025.1
• Coordinates: 35078..35326 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF70_RS25590	32445..33014	-	570	WP_004152117.1	small heat shock protein sHSP20	-
JLF70_RS25595	33049..33330	-	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
JLF70_RS25600	33574..33837	-	264	WP_004118208.1	hypothetical protein	-
JLF70_RS25605	33852..34115	+	264	WP_004187027.1	transposase	-
JLF70_RS25610	34060..34176	+	117	Protein_34	transposase domain-containing protein	-
JLF70_RS25615	34364..34788	+	425	Protein_35	IS1 family transposase	-
JLF70_RS25620	35078..35326	+	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
JLF70_RS25625	35316..35600	+	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF70_RS25630	35616..36971	-	1356	WP_004187017.1	ISNCY family transposase	-
JLF70_RS25635	37053..37352	-	300	Protein_39	conjugal transfer protein TraH	-
JLF70_RS25640	37350..37833	-	484	Protein_40	hypothetical protein	-
JLF70_RS25645	37850..38374	-	525	Protein_41	hypothetical protein	-
JLF70_RS25650	39917..40381	+	465	WP_004187010.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..142386	142386
	-	flank	IS/Tn	-	-	34558..34824	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T35962 WP_004187019.1 NZ_AP022528:35316-35600 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T35962 NZ_AP022528:35316-35600 [Klebsiella pneumoniae]
ATGACCTATAAGCTGGCTTTCAACGAATCCGCTCTGAAAGAATGGAAGAAGCTGGGTCATACACTACAGGTACAGTTCAA
AAAGAAACTCAAAGAGCGCCTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGATATCGCCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTTATCGGCGTAGGCAAA
CGCGAAAACGACGACATTTATAATGCAACCCTTAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT35962 WP_004187025.1 NZ_AP022528:35078-35326 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT35962 NZ_AP022528:35078-35326 [Klebsiella pneumoniae]
ATGGCATTTCAGGTTCTGACCACTACAGCAGCTAGCATTACTGAACTTAAACGTGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGTGTGCCACCGGCACTCTATGCCCAGTTGA
TGGAGCTTCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGAGAAAAGGTTATAGAGGTCAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4