Detailed information of TA system TA035957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4562357..4563167
• Replicon: chromosome
• Accession: NZ_AP022527
• Organism: Klebsiella pneumoniae strain THO-004

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: JLF70_RS22295
• Protein ID: WP_023343052.1
• Coordinates: 4562357..4562890 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: JLF70_RS22300
• Protein ID: WP_023343051.1
• Coordinates: 4562901..4563167 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF70_RS22290	4561188..4562309	+	1122	WP_012737335.1	cupin domain-containing protein	-
JLF70_RS22295	4562357..4562890	-	534	WP_023343052.1	GNAT family N-acetyltransferase	Toxin
JLF70_RS22300	4562901..4563167	-	267	WP_023343051.1	DUF1778 domain-containing protein	Antitoxin
JLF70_RS22305	4563270..4564703	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
JLF70_RS22310	4564693..4565376	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
JLF70_RS22315	4565549..4566934	+	1386	WP_024623207.1	efflux transporter outer membrane subunit	-
JLF70_RS22320	4566952..4567296	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19825.70 Da
• Isoelectric Point: 4.9881

>T35957 WP_023343052.1 NZ_AP022527:c4562890-4562357 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKWQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T35957 NZ_AP022527:c4562890-4562357 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGTGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCTGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT35957 WP_023343051.1 NZ_AP022527:c4563167-4562901 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT35957 NZ_AP022527:c4563167-4562901 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
GGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA