Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3946633..3947252 Replicon chromosome
Accession NZ_AP022527
Organism Klebsiella pneumoniae strain THO-004

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JLF70_RS19315 Protein ID WP_002892050.1
Coordinates 3947034..3947252 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag JLF70_RS19310 Protein ID WP_002892066.1
Coordinates 3946633..3947007 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF70_RS19300 3941785..3942978 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JLF70_RS19305 3943001..3946147 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
JLF70_RS19310 3946633..3947007 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
JLF70_RS19315 3947034..3947252 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
JLF70_RS19320 3947411..3947977 + 567 WP_002892030.1 maltose O-acetyltransferase -
JLF70_RS19325 3947949..3948089 - 141 WP_004147370.1 hypothetical protein -
JLF70_RS19330 3948110..3948580 + 471 WP_002892026.1 YlaC family protein -
JLF70_RS19335 3948555..3950006 - 1452 WP_023302231.1 PLP-dependent aminotransferase family protein -
JLF70_RS19340 3950107..3950805 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
JLF70_RS19345 3950802..3950942 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
JLF70_RS19350 3950942..3951205 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35956 WP_002892050.1 NZ_AP022527:3947034-3947252 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T35956 NZ_AP022527:3947034-3947252 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT35956 WP_002892066.1 NZ_AP022527:3946633-3947007 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT35956 NZ_AP022527:3946633-3947007 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References