Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58472..58997 Replicon plasmid pTHO-003-1
Accession NZ_AP022526
Organism Escherichia coli strain THO-003

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag JLF28_RS25950 Protein ID WP_001159871.1
Coordinates 58692..58997 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag JLF28_RS25945 Protein ID WP_000813630.1
Coordinates 58472..58690 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF28_RS25920 54545..55249 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
JLF28_RS25925 55303..55644 - 342 Protein_59 DNA helicase UvrD -
JLF28_RS25930 55629..57194 - 1566 WP_001128474.1 AAA family ATPase -
JLF28_RS25935 57269..57685 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
JLF28_RS25940 57682..57912 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
JLF28_RS25945 58472..58690 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
JLF28_RS25950 58692..58997 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
JLF28_RS25955 58998..59804 + 807 WP_000016970.1 site-specific integrase -
JLF28_RS25960 60526..61281 + 756 WP_000852146.1 replication initiation protein RepE -
JLF28_RS25965 61869..63035 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..123467 123467


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T35939 WP_001159871.1 NZ_AP022526:58692-58997 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T35939 NZ_AP022526:58692-58997 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT35939 WP_000813630.1 NZ_AP022526:58472-58690 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT35939 NZ_AP022526:58472-58690 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References