Detailed information of TA system TA035933

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4412277..4413112
• Replicon: chromosome
• Accession: NZ_AP022525
• Organism: Escherichia coli strain THO-003

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: JLF28_RS21665
• Protein ID: WP_000854759.1
• Coordinates: 4412277..4412654 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: JLF28_RS21670
• Protein ID: WP_001295723.1
• Coordinates: 4412744..4413112 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF28_RS21635	4408215..4408391	-	177	Protein_4245	helix-turn-helix domain-containing protein	-
JLF28_RS21640	4408583..4409329	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
JLF28_RS21645	4409344..4410885	-	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
JLF28_RS21650	4411341..4411499	-	159	WP_001467148.1	hypothetical protein	-
JLF28_RS21655	4411599..4411775	-	177	WP_000839286.1	DUF957 domain-containing protein	-
JLF28_RS21660	4411792..4412280	-	489	WP_000761690.1	hypothetical protein	-
JLF28_RS21665	4412277..4412654	-	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
JLF28_RS21670	4412744..4413112	-	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF28_RS21675	4413275..4413496	-	222	WP_000692345.1	DUF987 domain-containing protein	-
JLF28_RS21680	4413559..4414035	-	477	WP_001186775.1	RadC family protein	-
JLF28_RS21685	4414051..4414524	-	474	WP_001350782.1	antirestriction protein	-
JLF28_RS21690	4414866..4415684	-	819	WP_001234738.1	DUF945 domain-containing protein	-
JLF28_RS21695	4415839..4415997	-	159	WP_001323397.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4402867..4427650	24783

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T35933 WP_000854759.1 NZ_AP022525:c4412654-4412277 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T35933 NZ_AP022525:c4412654-4412277 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT35933 WP_001295723.1 NZ_AP022525:c4413112-4412744 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT35933 NZ_AP022525:c4413112-4412744 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52